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解析从孟加拉国女士大厅污水中分离出的多重耐药菌株哈基姆RU_GHWS的全基因组序列。

Deciphering the complete genome sequence of multidrug-resistant strain Hakim RU_GHWS isolated from ladies' hall sewage water in Bangladesh.

作者信息

Polash Md Arif-Uz-Zaman, Zahan Nusrat, Khan Muhib Ullah, Sarker Subir, Zhang Tianyu, Haque Md Hakimul

机构信息

Department of Veterinary and Animal Sciences, University of Rajshahi, Rajshahi, Bangladesh.

Biomedical Sciences & Molecular Biology, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, Australia.

出版信息

Microbiol Resour Announc. 2024 Oct 10;13(10):e0074124. doi: 10.1128/mra.00741-24. Epub 2024 Sep 16.

DOI:10.1128/mra.00741-24
PMID:39283155
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11465733/
Abstract

This report details the genome sequence of strain Hakim RU_GHWS, isolated from sewage water. The assembled genome comprises 5.022 Mb with 77.675× coverage, depicting an average GC content of 50.50%. This genome contains 10 CRISPR arrays, 14 prophages, 65 antibiotic resistance genes, and 28 virulence factor genes.

摘要

本报告详细介绍了从污水中分离出的哈基姆RU_GHWS菌株的基因组序列。组装后的基因组包含5.022兆碱基对，覆盖度为77.675倍，平均GC含量为50.50%。该基因组包含10个CRISPR阵列、14个原噬菌体、65个抗生素抗性基因和28个毒力因子基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a8/11465733/eff2da32cedf/mra.00741-24.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a8/11465733/eff2da32cedf/mra.00741-24.f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1a8/11465733/eff2da32cedf/mra.00741-24.f001.jpg

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本文引用的文献

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Extended-spectrum beta-lactamase in isolated from humans, animals, and environments in Bangladesh: A One Health perspective systematic review and meta-analysis.孟加拉国人类、动物和环境中分离出的超广谱β-内酰胺酶：“同一健康”视角的系统评价与荟萃分析
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CRISPRimmunity: an interactive web server for CRISPR-associated Important Molecular events and Modulators Used in geNome edIting Tool identifYing.
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Nucleic Acids Res. 2023 Jul 5;51(W1):W93-W107. doi: 10.1093/nar/gkad425.
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An SPRI beads-based DNA purification strategy for flexibility and cost-effectiveness.基于 SPRI 磁珠的 DNA 纯化策略，兼具灵活性和成本效益。
BMC Genomics. 2023 Mar 16;24(1):125. doi: 10.1186/s12864-023-09211-w.
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ResFinder - an open online resource for identification of antimicrobial resistance genes in next-generation sequencing data and prediction of phenotypes from genotypes.ResFinder-一个用于鉴定下一代测序数据中抗菌药物耐药基因和从基因型预测表型的开放在线资源。
Microb Genom. 2022 Jan;8(1). doi: 10.1099/mgen.0.000748.
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VFDB 2022: a general classification scheme for bacterial virulence factors.VFDB 2022：细菌毒力因子的通用分类方案。
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