三抗生素糊剂对牙本质小管内粪肠球菌杀菌效果的体外研究。

Bactericidal Effect of Triple Antibiotic Paste against Enterococcus faecalis in Dentinal Tubules: An Ex Vivo Study.

机构信息

Department of Operative Dentistry and Endodontics, Mahidol University Faculty of Dentistry, Bangkok, Thailand.

Department of Oral Microbiology, Mahidol University Faculty of Dentistry, Bangkok, Thailand.

出版信息

Eur Endod J. 2024 Mar;9(2):161-166. doi: 10.14744/eej.2023.78942.

DOI:10.14744/eej.2023.78942

PMID:38456464

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10938359/

Abstract

OBJECTIVE

The aim of this study was to investigate the bactericidal effect of various concentrations of triple antibiotic paste (TAP) against Enterococcus faecalis (E. faecalis) in dentinal tubules using a bacterial culture assay and confocal laser scanning microscope (CLSM).

METHODS

Ninety human teeth were contaminated with E. faecalis (ATCC 29212) and randomly allocated into 5 groups; the negative control (without TAP), 1 mg/ml, 5 mg/ml, 7.5 mg/ml and 10 mg/ml TAP (n=18). After a 3-week TAP treatment, samples were collected from the root canal space, root dentin at 100-μm and 200-μm depth. The collected samples were subjected to a bacterial culture assay (n=10). Eight roots from each group underwent CLSM analysis to determine the live/dead bacterial cells.

RESULTS

The bacterial culture assay results indicated that the negative control samples were all culturable. The number of culture-positive samples decreased after TAP treatment at 1, 5, 7.5 and 10 mg/ml, with 2, 2, 1 and 0 culturable samples, respectively. However, there was no significant difference among the TAP treatments. Surprisingly, the CLSM analysis demonstrated live bacteria in the dentinal tubules in all samples. The negative control had 52.36%+-3.24 live bacteria. TAP treatment at 10 mg/ml had the lowest percentage of live bacterial cells (40.58%+-5.40), followed by 7.5 mg/ml (44.14%+-6.03), 5 mg/ml (46.31%+-5.32) and 1 mg/ml (52.55%+-8.82). The percentage of live cells in the 10 mg/ml, 7.5 mg/ml and 5 mg/ml TAP groups were significantly lower than the 1 mg/ml TAP and negative control groups.

CONCLUSION

TAP treatment significantly decreased the percentage of viable E. faecalis cells in the dentinal tubules and its bactericidal effect was dose-dependent.

摘要

目的

本研究旨在通过细菌培养实验和共聚焦激光扫描显微镜（CLSM）研究不同浓度三联抗生素糊剂（TAP）对牙本质小管内粪肠球菌（E. faecalis）的杀菌效果。

方法

将 90 个人工污染粪肠球菌（ATCC 29212）的牙齿随机分为 5 组，阴性对照组（无 TAP）、1 mg/ml、5 mg/ml、7.5 mg/ml 和 10 mg/ml TAP 组（每组 18 个样本）。经过 3 周的 TAP 处理后，从根管空间、100-μm 和 200-μm 深度的根管牙本质采集样本。收集的样本进行细菌培养实验（n=10）。每组 8 个样本进行 CLSM 分析以确定活/死细菌细胞。

结果

细菌培养实验结果表明，阴性对照组的所有样本均有培养活性。TAP 处理后，1、5、7.5 和 10 mg/ml 组的培养阳性样本数分别减少至 2、2、1 和 0 个，但 TAP 处理之间无显著差异。令人惊讶的是，CLSM 分析显示所有样本的牙本质小管内均有活细菌。阴性对照组有 52.36%+-3.24 个活细菌。TAP 处理 10 mg/ml 组的活细菌细胞百分比最低（40.58%+-5.40），其次是 7.5 mg/ml（44.14%+-6.03）、5 mg/ml（46.31%+-5.32）和 1 mg/ml（52.55%+-8.82）。10 mg/ml、7.5 mg/ml 和 5 mg/ml TAP 组的活细胞百分比显著低于 1 mg/ml TAP 组和阴性对照组。