Kucuk Meltem, Quevedo Grace, Ratakonda Mohini, Aksel Hacer
Department of Endodontics, Faculty of Dentistry, Near East University, Mersin, Turkey.
Department of Periodontics and Endodontics, School of Dental Medicine, University at Buffalo, Buffalo, NY, USA.
Sci Rep. 2025 Jul 2;15(1):23581. doi: 10.1038/s41598-025-09057-0.
This study evaluated the antibacterial and biocompatibility effects of Gelatin Methacrylate Hydrogel (GelMA) loaded with a double antibiotic paste (DAP) and bioactive glass (BG). The cellular impact of 1 mg/mL (1DAP) and 5 mg/mL (5DAP) DAP, as well as 1% BG (1BG) and 10% BG (10BG) within the hydrogel on dental pulp stem cells (DPSCs), was assessed using ATP cell viability and alkaline phosphatase (ALP) assays over 21 days. Antibacterial efficacy against E. faecalis was measured with and without dentin disks after 24-hour incubation, followed by colony-forming unit (CFU) counting. The statistical analysis was performed using one- and two-way ANOVA and Kruskal-Wallis tests. Results indicated that 5DAP and 10BG significantly reduced the vitality of DPSCs compared to 1DAP, 1BG, GelMA alone, and the control group (p < 0.001). Conversely, 1DAP and 1BG demonstrated the highest cell viability, with 1BG exhibiting superior ALP activity (p < 0.001). In the absence of dentin disks, 10BG and 5DAP displayed the greatest antibacterial effects (p < 0.001). However, the presence of dentin significantly diminished the antibacterial efficacy of BG, with 5DAP showing the strongest effect on infected dentin disks (p = 0.008). GelMA with 1% BG enhanced cellular functions. Dentin significantly reduced the antibacterial efficacy of BG, regardless of concentration. DAP at 5 mg/mL provided the highest antibacterial activity on infected dentin disks. However, none of the hydrogels completely eliminated bacteria from the dentin disks.
本研究评估了负载双抗生素糊剂（DAP）和生物活性玻璃（BG）的甲基丙烯酸明胶水凝胶（GelMA）的抗菌和生物相容性作用。使用ATP细胞活力和碱性磷酸酶（ALP）测定法，在21天内评估水凝胶中1mg/mL（1DAP）和5mg/mL（5DAP）的DAP以及1%BG（1BG）和10%BG（10BG）对牙髓干细胞（DPSC）的细胞影响。在孵育24小时后，通过有和没有牙本质盘的情况下对粪肠球菌的抗菌效力进行测量，随后进行菌落形成单位（CFU）计数。使用单因素和双因素方差分析以及Kruskal-Wallis检验进行统计分析。结果表明，与1DAP、1BG、单独的GelMA和对照组相比，5DAP和10BG显著降低了DPSC的活力（p<0.001）。相反，1DAP和1BG表现出最高的细胞活力，1BG表现出更高的ALP活性（p<0.001）。在没有牙本质盘的情况下，10BG和5DAP显示出最大的抗菌效果（p<0.001）。然而，牙本质的存在显著降低了BG的抗菌效力，5DAP对感染的牙本质盘显示出最强的效果（p=0.008）。含1%BG的GelMA增强了细胞功能。无论浓度如何，牙本质均显著降低了BG的抗菌效力。5mg/mL的DAP对感染的牙本质盘具有最高的抗菌活性。然而，没有一种水凝胶能完全消除牙本质盘中的细菌。
