Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Aust Endod J. 2023 Sep;49 Suppl 1:9-17. doi: 10.1111/aej.12683. Epub 2022 Sep 8.
The aim of this study was to evaluate the bactericidal efficacy on Enterococcus faecalis biofilm of a novel, low-concentration triple-antibiotic paste (TAP-L2) in propylene glycol/carboxymethylcellulose vehicle (VEH-2), a ready-to-use prototype, compared with two currently used products; a low-concentration (TAP-L1) and a high-concentration paste (TAP-H1) mixed in macrogol/propylene glycol vehicle (VEH-1). Sixty-two root canals were infected with E. faecalis biofilm, medicated with (a) TAP-L1, (b) TAP-L2, (c) TAP-H1, (d) VEH-1 or (e) VEH-2 (n = 10) and incubated for 21 days. Live/dead assays were performed using confocal laser scanning microscopy (CLSM), and dead bacteria (%) were calculated. The TAP-H1 group demonstrated the highest dead bacteria (67.54 ± 2.38%), which was significantly higher than the TAP-L2 and TAP-L1 groups (56.85 ± 7.11% and 54.23 ± 10.19%) (p < 0.05). The TAP groups demonstrated significantly higher dead bacteria than the VEH-1 and VEH-2 groups (47.51 ± 6.41% and 45.14 ± 8.28%) (p < 0.05). The ready-to-use TAP-L2 had antibacterial activity comparable to TAP-L1, both of which were lower than TAP-H1.
本研究旨在评估载于丙二醇/羧甲基纤维素载体（VEH-2）中的新型低浓度三联抗生素糊剂（TAP-L2）的杀菌功效，该糊剂为即用型原型制剂，与两种现有产品相比：一种低浓度（TAP-L1）和一种高浓度糊剂（TAP-H1）混合于聚乙二醇/丙二醇载体（VEH-1）中。62 个根管感染粪肠球菌生物膜，用（a）TAP-L1、（b）TAP-L2、（c）TAP-H1、（d）VEH-1 或（e）VEH-2（n=10）处理，并孵育 21 天。使用共聚焦激光扫描显微镜（CLSM）进行活/死检测，并计算死菌（%）。TAP-H1 组的死菌（67.54±2.38%）最高，明显高于 TAP-L2 和 TAP-L1 组（56.85±7.11%和 54.23±10.19%）（p<0.05）。TAP 组的死菌明显高于 VEH-1 和 VEH-2 组（47.51±6.41%和 45.14±8.28%）（p<0.05）。即用型 TAP-L2 的抗菌活性与 TAP-L1 相当，均低于 TAP-H1。
