Frough Reyhani Mohammad, Rahimi Saeed, Fathi Zahra, Shakouie Sahar, Salem Milani Amin, Soroush Barhaghi Mohammad Hossein, Shokri Javad
Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Associate Professor, Department of Endodontic, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ; Professor, Department of Endodontic, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
J Dent Res Dent Clin Dent Prospects. 2015 Summer;9(3):138-43. doi: 10.15171/joddd.2015.027. Epub 2015 Sep 16.
Background and aims. Triple antibiotic paste (TAP) is widely used in endodontics for root canal disinfection, particularly in regenerative procedures. The aim of this in vitro study was to evaluate the antimicrobial effects of different concentrations of TAP at 1-, 2-, 3-, and 4-week intervals on mature Enterococcus faecalis biofilm. Materials and methods. A total of 287 extracted one-rooted human central incisors were infected with E. faecalis ATCC 29212 after removing the crown and preparation. The root canal space was filled with one of the 0.01-, 0.1-, 1-, 10-, 100-, and 1000-mg/mL concentrations of TAP or normal saline (control). The root canal dentin was sampled after 1, 2, 3, and 4 weeks. The dentinal shavings were cultured on Mueller-Hinton agar plates after serial dilutions. The classic colony-forming unit (CFU) counting technique was used to determine remaining bacterial counts. Data were analyzed by using the two-way ANOVA, post hoc Tukey tests and one-way ANOVA (P<0.05). Results. TAP completely eliminated E. faecalis biofilms at all the intervals at concentrations of 1000, 100, and 10 mg/mL, whereas 1-, 0.1-, and 0.01-mg/mL TAP resulted in significant reduction of CFU means compared with the control group. There were no statistically significant differences between the four time intervals. Conclusion. Use of lower concentrations of TAP at short term could eradicate E. faecalis biofilm and decrease high-concentration side effects.
背景与目的。三联抗生素糊剂（TAP）在牙髓病学中广泛用于根管消毒，尤其是在再生治疗中。本体外研究的目的是评估不同浓度的TAP在1、2、3和4周时间间隔对成熟粪肠球菌生物膜的抗菌作用。材料与方法。总共287颗拔除的单根人正中切牙在去除牙冠并预备后感染粪肠球菌ATCC 29212。根管空间用0.01、0.1、1、10、100和1000mg/mL浓度的TAP或生理盐水（对照组）之一充填。在1、2、3和4周后采集根管牙本质样本。牙本质碎屑经系列稀释后在穆勒-欣顿琼脂平板上培养。采用经典的菌落形成单位（CFU）计数技术确定残留细菌数量。数据采用双向方差分析、事后Tukey检验和单向方差分析进行分析（P<0.05）。结果。在1000、100和10mg/mL浓度下，TAP在所有时间间隔均完全消除了粪肠球菌生物膜，而1、0.1和0.01mg/mL的TAP与对照组相比导致CFU平均值显著降低。四个时间间隔之间无统计学显著差异。结论。短期使用较低浓度的TAP可根除粪肠球菌生物膜并降低高浓度的副作用。
