Shiomi T, Sato K
Somatic Cell Genet. 1979 Mar;5(2):193-201. doi: 10.1007/BF01539160.
A new method (viral suicide method) for the isolation of UV-sensitive mutants is described. Colonies of mutagenized human FL cells were infected with UV-irradiated Herpes simplex viruses and surviving ones which seemed to be deficient in host cell reactivation (HCR) were examined for their UV sensitivity. Nineteen of 238 clones examined were sensitive to UV irradiation at the time of the isolation. After recloning, four of these clones have been studied and two (UVS-1 and UVS-2) of them are stable in their UV sensitivity for 4 months in culture. UV sensitivity of UVS-1, UVS-2, and the parental FL cells are as follows: the extrapolation numbers (n) are 2.2, 2.1, and 1.8 and mean lethal doses (D0) are 2.9, 3.7, and 7.8 J/m2 for UVS-1, UVS-2, and the parental FL cells, respectively- They are no more sensitive than FL cells to X-irradiation. The ability of HCR in UVS-2 cells is apparently lower than that in FL cells, whereas UVS-1 cells are the same as FL cells in the ability.
描述了一种分离紫外线敏感突变体的新方法（病毒自杀法）。用紫外线照射的单纯疱疹病毒感染诱变的人FL细胞集落，检查那些似乎缺乏宿主细胞再激活（HCR）的存活细胞的紫外线敏感性。在分离时，所检查的238个克隆中有19个对紫外线照射敏感。再克隆后，对其中4个克隆进行了研究，其中2个克隆（UVS - 1和UVS - 2）在培养4个月期间其紫外线敏感性保持稳定。UVS - 1、UVS - 2和亲本FL细胞的紫外线敏感性如下：UVS - 1、UVS - 2和亲本FL细胞的外推数（n）分别为2.2、2.1和1.8，平均致死剂量（D0）分别为2.9、3.7和7.8 J/m2。它们对X射线照射的敏感性并不比FL细胞更高。UVS - 2细胞的HCR能力明显低于FL细胞，而UVS - 1细胞在该能力方面与FL细胞相同。
