Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Bihar Animal Sciences University, Kishanganj-855107, Bihar, India.
Division of Surgery, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.
J Wound Care. 2024 Mar 1;33(Sup3a):lxxiv-lxxx. doi: 10.12968/jowc.2024.33.Sup3a.lxxiv.
The purpose of the study was to compare the healing potential of bubaline small intestinal matrix (bSIM) and fish swim bladder matrix (FSBM) on full-thickness skin wounds in rabbits.
Four full-thickness skin wounds (each 20×20mm) were created on the dorsum of 18 rabbits that were divided into three groups based on treatment: untreated sham control (I), implanted with double layers of bSIM (II) and implanted with double layers of FSBM (III). Macroscopic, immunologic and histologic observations were made to evaluate wound healing.
Gross healing progression in the bSIM and FSBM groups showed significantly (p<0.05) less wound contraction compared with the sham group. The IgG concentration in rabbit sera was significantly (p<0.05) lower in the FSBM group compared with the bSIM group by enzyme-linked immunosorbent assay. The stimulation index of peripheral blood lymphocytes was significantly (p<0.05) lower in the FSBM group compared with the bSIM group by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Implantation of FSBM resulted in improved re-epithelialisation, neovascularisation and fibroplasia.
The FSBM is a more effective dermal substitute when compared with the bSIM for full-thickness skin wound repair in rabbit.
本研究旨在比较努比亚小场肠基质（bSIM）和鱼鳔基质（FSBM）在兔全层皮肤创面愈合中的潜力。
在 18 只兔子的背部创建了 4 个全层皮肤创面（每个创面 20×20mm），根据治疗方法将兔子分为三组：未处理的假对照（I）组、植入双层 bSIM（II）组和植入双层 FSBM（III）组。进行宏观、免疫和组织学观察以评估伤口愈合。
bSIM 和 FSBM 组的大体愈合进展显示与假对照（sham）组相比，伤口收缩明显（p<0.05）减少。酶联免疫吸附试验（ELISA）显示，FSBM 组兔血清中的 IgG 浓度明显（p<0.05）低于 bSIM 组。MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐）试验显示，FSBM 组的外周血淋巴细胞刺激指数明显（p<0.05）低于 bSIM 组。FSBM 的植入促进了再上皮化、新生血管形成和纤维化。
与 bSIM 相比，FSBM 是一种更有效的真皮替代物，可用于兔全层皮肤创面修复。
