Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
Department of Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Provincial Clinical Research Center for Critical Care Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Institute of Molecular Precision Medicine and Hunan Key Laboratory of Molecular Precision Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China; Hunan Key Laboratory of Animal Models for Human Diseases, School of Life Sciences, Central South University, Changsha, Hunan 410008, China.
STAR Protoc. 2024 Mar 15;5(1):102942. doi: 10.1016/j.xpro.2024.102942. Epub 2024 Mar 7.
Septic cardiomyopathy is associated with high mortality in septic patients, characterized by reversible systolic and diastolic dysfunction. It is essential to monitor cardiac function and hemodynamic changes in septic animals. Here, we present a protocol to monitor cardiac function and hemodynamics in septic rodents. We describe steps for performing cecal ligation and puncture on rodents to induce sepsis, acquiring two-dimensional echocardiographic and M-mode ultrasonic images, and assessing mean arterial pressure in septic animals. For complete details on the use and execution of this protocol, please refer to Zhang et al..
脓毒症性心肌病与脓毒症患者的高死亡率相关，其特征为收缩和舒张功能可逆性障碍。监测脓毒症动物的心脏功能和血流动力学变化至关重要。在此，我们提供了监测脓毒症啮齿动物心脏功能和血流动力学的方案。我们描述了在啮齿动物上进行盲肠结扎和穿刺以诱导脓毒症、获取二维超声心动图和 M 型超声图像以及评估脓毒症动物平均动脉压的步骤。有关该方案使用和实施的完整详细信息，请参阅 Zhang 等人的研究。
