College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Department of Animal Sciences, Division of Nutritional Sciences, University of Illinois, Urbana, IL, 61801, USA.
BMC Vet Res. 2024 Mar 8;20(1):88. doi: 10.1186/s12917-024-03929-9.
Strontium (Sr) has similar physicochemical properties as calcium (Ca) and is often used to evaluate the absorption of this mineral. Because the major route of Ca absorption in the bovine occurs in the rumen, it is essential to understand whether Sr impacts the ruminal epithelial cells and to what extent.
In the present study, RNA sequencing and assembled transcriptome assembly were used to identify transcription factors (TFs), screening and bioinformatics analysis in bovine ruminal epithelial cells treated with Sr. A total of 1405 TFs were identified and classified into 64 families based on an alignment of conserved domains. A total of 174 differently expressed TFs (DE-TFs) were increased and 52 DE-TFs were decreased; the biological process-epithelial cell differentiation was inhibited according to the GSEA-GO analysis of TFs; The GO analysis of DE-TFs was enriched in the DNA binding. Protein-protein interaction network (PPI) found 12 hubs, including SMAD4, SMAD2, SMAD3, SP1, GATA2, NR3C1, PPARG, FOXO1, MEF2A, NCOA2, LEF1, and ETS1, which verified genes expression levels by real-time PCR.
In this study, SMAD2, PPARG, LEF1, ETS1, GATA2, MEF2A, and NCOA2 are potential candidates that could be targeted by Sr to mediate cell proliferation and differentiation, as well as lipid metabolism. Hence, these results enhance the comprehension of Sr in the regulation of transcription factors and provide new insight into the study of Sr biological function in ruminant animals.
锶（Sr）具有与钙（Ca）相似的理化性质，常被用于评估这种矿物质的吸收。由于牛对 Ca 的主要吸收途径发生在瘤胃中，因此了解 Sr 是否会影响瘤胃上皮细胞及其影响程度至关重要。
本研究采用 RNA 测序和组装转录组组装技术，筛选并对用 Sr 处理的牛瘤胃上皮细胞进行了生物信息学分析，共鉴定出 1405 个转录因子（TFs），并根据保守结构域比对将其分为 64 个家族。共鉴定出 174 个差异表达的 TFs（DE-TFs），其中 174 个上调，52 个下调；根据 TF 的 GSEA-GO 分析，生物学过程——上皮细胞分化受到抑制；GO 分析表明，DE-TFs 富集在 DNA 结合。蛋白质-蛋白质相互作用网络（PPI）发现了 12 个枢纽，包括 SMAD4、SMAD2、SMAD3、SP1、GATA2、NR3C1、PPARG、FOXO1、MEF2A、NCOA2、LEF1 和 ETS1，通过实时 PCR 验证了这些基因的表达水平。
本研究表明，SMAD2、PPARG、LEF1、ETS1、GATA2、MEF2A 和 NCOA2 可能是 Sr 介导细胞增殖和分化以及脂质代谢的潜在靶点。因此，这些结果增强了我们对 Sr 调节转录因子的理解，并为研究 Sr 在反刍动物中的生物学功能提供了新的视角。
