Raza Sayed Haidar Abbas, Pant Sameer D, Wani Atif Khurshid, Mohamed Hadeer H, Khalifa Norhan E, Almohaimeed Hailah M, Alshanwani Aliah R, Assiri Rasha, Aggad Waheeb S, Noreldin Ahmed E, Abdelnour Sameh A, Wang Zhe, Zan Linsen
College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, PR China.
School of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW, Australia.
Mol Cell Probes. 2022 Oct;65:101850. doi: 10.1016/j.mcp.2022.101850. Epub 2022 Aug 18.
Intramuscular fat (IMF) content is a crucial determinant of meat quality traits in livestock. A network of transcription factors act in concert to regulate adipocyte formation and differentiation, which in turn influences intramuscular fat. Several genes and associated transcription factors have been reported to influence lipogenesis and adipogenesis during fetal and subsequent growth stage. Specifically in cattle, Krüppel-like factors (KLFs), which represents a family of transcription factors, have been reported to be involved in adipogenic differentiation and development. KLFs are a relatively large group of zinc-finger transcription factors that have a variety of functions in addition to adipogenesis. In mammals, the participation of KLFs in cell development and differentiation is well known. Specifically in the context of adipogenesis, KLFs function either as positive (KLF4, KLF5, KLF6, KLF8, KLF9, KLF10, KLF11, KLF12, KLF13, KLF14 and KLF15) or negative organizers (KLF2, KLF3 and KLF7), by a variety of different mechanisms such as crosstalk with C/EBP and PPARγ. In this review, we aim to summarize the potential functions of KLFs in regulating adipogenesis and associated pathways in cattle. Furthermore, the function of known bovine adipogenic marker genes, and associated transcription factors that regulate the expression of these marker genes is also summarized. Overall, this review will provide an overview of marker genes known to influence bovine adipogenesis and regulation of expression of these genes, to provide insights into leveraging these genes and transcription factors to enhance breeding programs, especially in the context of IMF deposition and meat quality.
肌内脂肪（IMF）含量是家畜肉质性状的关键决定因素。转录因子网络协同作用以调节脂肪细胞的形成和分化，进而影响肌内脂肪。据报道，一些基因及相关转录因子在胎儿期及随后的生长阶段会影响脂肪生成和脂肪形成。具体而言，在牛中，Krüppel样因子（KLFs）是一个转录因子家族，已被报道参与脂肪生成分化和发育。KLFs是相对较大的一组锌指转录因子，除脂肪生成外还具有多种功能。在哺乳动物中，KLFs参与细胞发育和分化是众所周知的。具体在脂肪生成方面，KLFs通过与C/EBP和PPARγ等多种不同机制相互作用，作为正向（KLF4、KLF5、KLF6、KLF8、KLF9、KLF10、KLF11、KLF12、KLF13、KLF14和KLF15）或负向调节因子（KLF2、KLF3和KLF7）发挥作用。在本综述中，我们旨在总结KLFs在调节牛脂肪生成及相关途径中的潜在功能。此外，还总结了已知的牛脂肪生成标记基因的功能，以及调节这些标记基因表达的相关转录因子。总体而言，本综述将概述已知影响牛脂肪生成的标记基因及其表达调控，以期深入了解如何利用这些基因和转录因子来加强育种计划，特别是在肌内脂肪沉积和肉质方面。
