白藜芦醇纳米制剂通过诱导自噬抑制侵袭性乳腺癌细胞生长：一项研究。

Resveratrol Nanoformulation Inhibits Invasive Breast Cancer Cell Growth through Autophagy Induction: An Study.

作者信息

Khazaei Mohammad Rasool, Bozorgi Maryam, Khazaei Mozafar, Aftabi Maryam, Bozorgi Azam

机构信息

Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.

出版信息

Cell J. 2024 Feb 1;26(2):112-120. doi: 10.22074/cellj.2024.2016930.1458.

DOI:10.22074/cellj.2024.2016930.1458

PMID:38459728

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10924839/

Abstract

OBJECTIVE

The aim of this study was to synthesize chitosan nanoparticles (Cs NPs) for resveratrol (RSV) delivery and assess their effectiveness in inducing autophagy in MDA-MB 231 cells.

MATERIALS AND METHODS

In this experimental study, Pure and RSV-loaded Cs NPs (RSV. Cs NPs) were prepared via the ionic gelation method, and their physicochemical properties were characterized using standard techniques, and RSV release was measured . MDA-MB 231 cells were incubated with RSV, Cs NPs, and RSV. Cs NPs and Half-maximal inhibitory concentration (IC50) values were calculated following the MTT test. Cell viability was assessed by lactate dehydrogenase (LDH) assay, and autophagy was evaluated using the real-time polymerase chain reaction (PCR).

RESULTS

NP formation was confirmed with the analysis of FTIR spectra. Pure and RSV. Cs NPs had 36.7 and 94.07 nm sizes with 18.3 and 27 mV zeta potentials, respectively. Above 60% of RSV entrapped within NPs was released in an initial burst manner followed by a gradual release till 72 hours. Cs and RSV. Cs NPs restrained cell proliferation at lower concentrations. RSV. Cs NPs showed the highest anticancer effect and stimulated autophagy, indicated by increased Beclin-1 ATG5, ATG7, LC3A, and P62 expression.

CONCLUSION

RSV. Cs NPs show promising effects in inhibiting invasive breast cancer (BC) cells by inducing autophagy.

摘要

目的

本研究旨在合成用于白藜芦醇（RSV）递送的壳聚糖纳米颗粒（Cs NPs），并评估其在诱导MDA-MB 231细胞自噬方面的有效性。

材料与方法

在本实验研究中，通过离子凝胶法制备了纯的和负载RSV的Cs NPs（RSV.Cs NPs），使用标准技术对其理化性质进行了表征，并测定了RSV的释放情况。将MDA-MB 231细胞与RSV、Cs NPs和RSV.Cs NPs一起孵育，通过MTT试验计算半数抑制浓度（IC50）值。通过乳酸脱氢酶（LDH）测定评估细胞活力，并使用实时聚合酶链反应（PCR）评估自噬情况。

结果

通过傅里叶变换红外光谱（FTIR）分析证实了纳米颗粒的形成。纯的和RSV.Cs NPs的粒径分别为36.7和94.07 nm，zeta电位分别为18.3和27 mV。包裹在纳米颗粒中的RSV有60%以上在最初的爆发式释放中释放出来，随后逐渐释放直至72小时。Cs和RSV.Cs NPs在较低浓度下抑制细胞增殖。RSV.Cs NPs显示出最高的抗癌效果并刺激自噬，这表现为Beclin-1、ATG5、ATG7、LC3A和P62表达增加。