Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece; Marianthi Simou and G.P. Livanos Labs, 1st Department of Critical Care and Pulmonary Services, School of Medicine, National & Kapodistrian University of Athens, ''Evangelismos'' Hospital, 10676 Athens, Greece.
Molecular Carcinogenesis Group, Department of Histology and Embryology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece; Biomedical Research Foundation, Academy of Athens, 11527 Athens, Greece.
STAR Protoc. 2024 Mar 15;5(1):102929. doi: 10.1016/j.xpro.2024.102929. Epub 2024 Mar 8.
Identification and isolation of senescent cells is challenging, rendering their detailed analysis an unmet need. We describe a precise one-step protocol to fluorescently label senescent cells, for flow cytometry and fluorescence microscopy, implementing a fluorophore-conjugated Sudan Black-B analog, GLF16. Also, a micelle-based approach allows identification of senescent cells in vivo and in vitro, enabling live-cell sorting for downstream analyses and live in vivo tracking. Our protocols are applicable to cellular systems, tissues, or animal models where senescence is present. For complete details on the use and execution of this protocol, please refer to Magkouta et al..
鉴定和分离衰老细胞具有挑战性，因此详细分析它们是未满足的需求。我们描述了一种精确的一步法方案，使用荧光标记的苏丹黑 B 类似物 GLF16 对衰老细胞进行荧光标记，用于流式细胞术和荧光显微镜术。此外，基于胶束的方法可以鉴定体内和体外的衰老细胞，实现活细胞分选，用于下游分析和活体内追踪。我们的方案适用于存在衰老的细胞系统、组织或动物模型。如需了解本方案的使用和实施的完整详细信息，请参阅 Magkouta 等人的文献。
