Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, Singapore 169857, Singapore.
Duke University School of Medicine, Durham, NC, USA.
STAR Protoc. 2020 Jul 1;1(2):100064. doi: 10.1016/j.xpro.2020.100064. eCollection 2020 Sep 18.
Previously developed senescent primary fibroblast models have limited relevance to study age-related changes in metabolically active tissues such as the liver. Here, we describe a protocol to generate senescent cells from the mouse hepatic cell line, AML12. These senescent cells exhibit molecular and metabolic signatures that are similar to those observed in livers from aged mice. These senescent AML12 cells should be a useful model to study the metabolic effects of aging in the liver. For complete details on the use and execution of this protocol, please refer to Singh et al. (2020).
先前开发的衰老原代成纤维细胞模型与研究代谢活跃组织（如肝脏）的年龄相关变化的相关性有限。在这里，我们描述了一种从鼠肝细胞系 AML12 中生成衰老细胞的方案。这些衰老细胞表现出与老年小鼠肝脏中观察到的分子和代谢特征相似的特征。这些衰老的 AML12 细胞应该是研究肝脏衰老代谢影响的有用模型。有关该方案的使用和执行的完整详细信息，请参阅 Singh 等人。（2020 年）。
