Environmental Biotechnology and Genomics Division, CSIR-National Environmental Engineering Research Institute, Nagpur 440020, India; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, India.
Environmental Biotechnology and Genomics Division, CSIR-National Environmental Engineering Research Institute, Nagpur 440020, India; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, India.
Bioresour Technol. 2024 May;399:130560. doi: 10.1016/j.biortech.2024.130560. Epub 2024 Mar 8.
The potential of hydrolytic enzyme cocktail obtained from designed bacterial consortium WSh-1 comprising Bacillus subtilis CRN 16, Paenibacillus dendritiformis CRN 18, Niallia circulans CRN 24, Serratia marscens CRN 29, and Streptomyces sp. CRN 30, was investigated for maximum saccharification. Activity was further enhanced to 1.01 U/ml from 0.82 U/ml by supplementing growth medium with biotin and cellobiose as a cofactor and inducer. Through kinetic analysis, the enzyme cocktail showed a high wheat straw affinity with Michaelis-Menten constant (Km) of 0.68 µmol/L and a deconstruction rate (Vmax) of 4.5 U/ml/min. The statistical optimization of critical parameters increased saccharification to 89 %. The optimized process in a 5-L lab-scale bioreactor yielded 501 mg/g of reducing sugar from NaOH-pretreated wheat straw. Lastly, genomic insights revealed unique abundant oligosaccharide deconstruction enzymes with the most diverse CAZyme profile. The consortium-mediated enzyme cocktails offer broader versatility with efficiency for the economical and sustainable valorization of lignocellulosic waste.
从由枯草芽孢杆菌 CRN 16、巨大芽胞杆菌 CRN 18、尼氏环链霉菌 CRN 24、沙雷氏菌 CRN 29 和链霉菌 CRN 30 组成的设计细菌联合体 WSh-1 获得的水解酶混合物的潜力进行了研究，以实现最大糖化。通过在生长培养基中添加生物素和纤维二糖作为辅因子和诱导物，将活性从 0.82 U/ml 进一步提高到 1.01 U/ml。通过动力学分析，该酶混合物对小麦秸秆具有很高的亲和力，米氏常数 (Km) 为 0.68 µmol/L，解构速率 (Vmax) 为 4.5 U/ml/min。关键参数的统计优化使糖化率提高到 89%。在 5-L 实验室规模生物反应器中的优化工艺从 NaOH 预处理的小麦秸秆中产生了 501 mg/g 的还原糖。最后，基因组分析揭示了独特的丰富寡糖解构酶，具有最多样的 CAZyme 谱。联合体介导的酶混合物提供了更广泛的多功能性和效率，用于经济和可持续地利用木质纤维素废物。
