Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea; Institute of SK-KU Biomaterials, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
Institute of SK-KU Biomaterials, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
Bioresour Technol. 2015 Mar;179:50-57. doi: 10.1016/j.biortech.2014.11.059. Epub 2014 Nov 20.
Two different biomasses were subjected to simultaneous pretreatment and saccharification (SPS) using a cocktail of hydrolytic and oxidizing enzymes. Application of a novel laccase as a detoxifying agent caused the removal of 49.8% and 32.6% of phenolic contents from the soaked rice straw and willow, respectively. Hydrolysis of soaked substrates using a newly developed fungal consortium resulted in saccharification yield of up to 74.2% and 63.6% for rice straw and willow, respectively. A high saccharification yield was obtained with soaked rice straw and willow without using any hazardous chemicals. The efficiency of each step related to SPS was confirmed by atomic force microscopy. The suitability of the developed SPS process was further confirmed by converting the hydrolysate from the process into bioethanol with 72.4% sugar conversion efficiency. To the best of our knowledge, this is the first report on the development of a less tedious, single-pot, and eco-friendly SPS methodology.
两种不同的生物质在水解和氧化酶混合物的同时预处理和糖化（SPS）下进行处理。新型漆酶作为解毒剂的应用分别导致浸泡的稻草和柳枝中的酚含量去除了 49.8%和 32.6%。使用新开发的真菌联合体对浸泡的底物进行水解，使稻草和柳枝的糖化产率分别高达 74.2%和 63.6%。未经任何危险化学品处理，即可获得高糖化产率的浸泡稻草和柳枝。原子力显微镜证实了与 SPS 相关的每个步骤的效率。通过将该工艺的水解产物转化为生物乙醇，转化率达到 72.4%，进一步证实了所开发的 SPS 工艺的适用性。据我们所知，这是首次报道开发出一种不繁琐、单罐、环保的 SPS 方法。
