Sun Jing-Yi, Li Cai, Du Feng-Ying
College of Basic Medical Science, Qilu Medical University, Zibo 255300, Shandong Province, China.
Department of Gastroenterological Surgery, Shandong Provincial Hospital of Shandong First Medical University, Jinan 250021, Shandong Province, China.
World J Orthop. 2024 Feb 18;15(2):192-200. doi: 10.5312/wjo.v15.i2.192.
The effectiveness of platelet-rich plasma (PRP) for the treatment of Achilles tendon disorders still needs to be evaluated through a series of prospective studies, but genomic analysis can reveal the existence of complementary PRP treatment options. Based on the 96 platelet activation-related genes in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, we performed Gene Ontology functional enrichment analysis and KEGG enrichment analysis, pathway correlation analysis, and enrichment mapping to determine the enrichment results of the gene set enrichment analysis and found that the cAMP signalling pathway may be the key to enhancing the effectiveness of PRP treatment. The cAMP signalling pathway interacts with the Rap1 signalling pathway and cGMP-PKG signalling pathway to mediate the entire pathophysiological process of Achilles tendon disease. Moreover, ADCY1-9 may be the key to the activation of the cAMP signalling network. Further based on the data in the Gene Expression Omnibus database, it was found that ADCY4 and ADCY7 may be the players that play a major role, associated with the STAT4-ADCY4-LAMA5 axis and the GRbeta-ADCY7-SEMA3C axis, which is expected to be a complementary target for enhancing the efficacy of PRP in the treatment of Achilles tendon disease.
富血小板血浆（PRP）治疗跟腱疾病的有效性仍需通过一系列前瞻性研究来评估，但基因组分析可以揭示PRP补充治疗方案的存在。基于京都基因与基因组百科全书（KEGG）数据库中的96个血小板活化相关基因，我们进行了基因本体功能富集分析、KEGG富集分析、通路相关性分析和富集映射，以确定基因集富集分析的富集结果，发现cAMP信号通路可能是提高PRP治疗效果的关键。cAMP信号通路与Rap1信号通路和cGMP-PKG信号通路相互作用，介导跟腱疾病的整个病理生理过程。此外，ADCY1-9可能是激活cAMP信号网络的关键。进一步基于基因表达综合数据库中的数据，发现ADCY4和ADCY7可能是起主要作用的因素，与STAT4-ADCY4-LAMA5轴和GRbeta-ADCY7-SEMA3C轴相关，有望成为增强PRP治疗跟腱疾病疗效的补充靶点。
