School of Life Science and Engineering, Foshan University, Foshan 528225, China.
School of Life Science and Engineering, Foshan University, Foshan 528225, China.
Poult Sci. 2021 Feb;100(2):1034-1048. doi: 10.1016/j.psj.2020.10.071. Epub 2020 Nov 19.
This study investigated the effect of berberine (BBR) on growth performance and composition and function of cecal microbiota in yellow-feathered broilers. A total of 360 1-day-old female broilers were assigned to 3 dietary treatments, each with 6 replicates of 20 birds. The dietary treatments consisted of a basal diet as negative control (NC), basal plus 200 mg/kg oxytetracycline calcium and 250 mg/kg nasiheptide as an antibiotic positive control (PC), and basal plus 250 mg/kg BBR. On day 21, 42, and 63, one chicken from each replicate was randomly selected for blood collection and cecal sampling. The 16S rRNA sequencing technology was used to analyze the community composition and function of cecal microbiota. Dietary supplementation with antibiotics or BBR increased the final body weight (BW) at day 63 and the average daily gain (ADG) during 1 to 21 d compared with the NC (P < 0.05). Supplementation with BBR improved the average daily feed intake (ADFI) at 22 to 42 d, 43 to 63 d, and 1 to 63 d (P < 0.05). Feed efficiency, indicated by feed to gain ratio (F/G), increased with PC during day 1 to 21 compared with NC (P < 0.05). The plasma concentrations of total protein at 42 d and uric acid at 21 d were increased, whereas creatine concentration at 63 d was decreased by BBR treatment (P < 0.05). The Chao 1 and Shannon index representing microbial α-diversity was reduced by BBR treatment (P < 0.05). The abundances of phylum Firmicutes and genera Lachnospiraceae, Lachnoclostridium, Clostridiales, and Intestinimonas were decreased, whereas the abundances of phylum Bacteroidetes and genus Bacteroides were increased with BBR treatment. Functional prediction of microbiota revealed that BBR treatment enriched pathways related to metabolism, organismal systems, and genetic information processing, especially DNA replication. The abundance of phylum Bacteroidetes, and genera Bacteroides and Lactobacillus in cecal contents were positively correlated with broiler growth performance. These results demonstrated dietary BBR supplementation improved the growth performance of yellow-feathered broilers, and was closely related to the significant changes in cecal microbiota composition.
本研究旨在探讨小檗碱（BBR）对黄羽肉鸡生长性能及盲肠微生物群落组成和功能的影响。将 360 只 1 日龄雌性肉鸡随机分为 3 个处理组，每个处理组设 6 个重复，每个重复 20 只鸡。对照组饲喂基础日粮，抗生素阳性对照组在基础日粮中添加 200 mg/kg 土霉素钙和 250 mg/kg 那西肽，BBR 阳性对照组在基础日粮中添加 250 mg/kg BBR。在第 21、42 和 63 天，每个重复随机选择 1 只鸡进行采血和盲肠采样。采用 16S rRNA 测序技术分析盲肠微生物群落组成和功能。与对照组相比，饲粮添加抗生素或 BBR 可提高第 63 天的末重（BW）和 121 日龄的平均日增重（ADG）（P<0.05）。BBR 可提高 2242 日龄、4363 日龄和 163 日龄的平均日采食量（ADFI）（P<0.05）。与对照组相比，饲粮添加抗生素可提高 1~21 日龄的料重比（F/G）（P<0.05）。BBR 处理可提高第 42 天的血浆总蛋白浓度和第 21 天的尿酸浓度，降低第 63 天的肌酸浓度（P<0.05）。BBR 处理可降低微生物 α-多样性的 Chao1 和 Shannon 指数（P<0.05）。BBR 处理可降低厚壁菌门和lachnospiraceae、lachnoclostridium、clostridiales 和 intestinimonas 属的丰度，增加拟杆菌门和 bacteroides 属的丰度。微生物群落功能预测显示，BBR 处理可富集与代谢、机体系统和遗传信息处理相关的途径，特别是 DNA 复制。盲肠内容物中厚壁菌门、bacteroides 属和 lactobacillus 属的丰度与肉鸡生长性能呈正相关。这些结果表明，饲粮补充 BBR 可改善黄羽肉鸡的生长性能，且与盲肠微生物群落组成的显著变化密切相关。
