Hong Changming, Huang Yujian, Yang Guan, Wen Xiaolu, Wang Li, Yang Xuefen, Gao Kaiguo, Jiang Zongyong, Xiao Hao
State Key Laboratory of Swine and Poultry Breeding Industry, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, China.
Anim Nutr. 2024 Jan 29;17:36-48. doi: 10.1016/j.aninu.2024.01.002. eCollection 2024 Jun.
Previous studies have shown that maternal resveratrol improved growth performance and altered the microbial composition of suckling piglets under hot summer conditions. However, it remains unclear how maternal resveratrol improves growth performance of suckling piglets during high summer temperatures. A total of 20 sows (Landrace × Large White; three parity) were randomly assigned to 2 groups (with or without 300 mg/kg resveratrol) from d 75 of gestation to d 21 of lactation during high ambient temperatures (from 27 to 30 °C). The results showed that maternal resveratrol supplementation increased total daily weight gain of piglets under hot summer conditions, which is consistent with previous studies. Furthermore, we found that maternal resveratrol improved the intestinal morphology and intestinal epithelial proliferation in suckling piglets. Dietary resveratrol supplementation affected the characteristics of exosome-derived microRNAs (miRNAs) in sow colostrum, as well as the genes targeted by differentially produced miRNAs. MiRNAs are concentrated in the tight junction pathway. As a result, the expression of intestinal tight junction proteins was increased in suckling piglets ( < 0.05). Notably, maternal resveratrol increased the intestinal secretory immunoglobulin A (sIgA) levels of suckling piglets via colostrum immunoglobin ( < 0.05), which could increase the abundance of beneficial microbiota to further increase the concentration of short chain fatty acids (SCFA) in suckling piglets' intestine ( < 0.05). Finally, our correlation analysis further demonstrated the positive associations between significantly differential intestinal microbiota, intestinal sIgA production and SCFA concentrations, as well as the positive relation between total daily weight gain and intestinal health of suckling piglets. Taken together, our findings suggested that maternal resveratrol could promote intestinal health to improve piglet growth during high summer temperatures, which might be associated with the immunoglobin and exosome-derived miRNAs in sows' colostrum.
先前的研究表明，在炎热的夏季条件下，母体补充白藜芦醇可改善哺乳仔猪的生长性能并改变其微生物组成。然而，母体白藜芦醇如何在夏季高温期间提高哺乳仔猪的生长性能仍不清楚。在高温环境（27至30°C）下，从妊娠第75天到哺乳第21天，将总共20头母猪（长白猪×大白猪；三胎）随机分为2组（添加或不添加300mg/kg白藜芦醇）。结果表明，在炎热的夏季条件下，母体补充白藜芦醇可增加仔猪的总日增重，这与先前的研究一致。此外，我们发现母体白藜芦醇可改善哺乳仔猪的肠道形态和肠道上皮细胞增殖。日粮中添加白藜芦醇会影响母猪初乳中源自外泌体的微小RNA（miRNA）的特征，以及差异产生的miRNA靶向的基因。miRNA集中在紧密连接途径中。结果，哺乳仔猪肠道紧密连接蛋白的表达增加（P<0.05）。值得注意的是，母体白藜芦醇通过初乳免疫球蛋白增加了哺乳仔猪的肠道分泌型免疫球蛋白A（sIgA）水平（P<0.05），这可以增加有益微生物群的丰度，从而进一步提高哺乳仔猪肠道中短链脂肪酸（SCFA）的浓度（P<0.05）。最后，我们的相关性分析进一步证明了显著差异的肠道微生物群、肠道sIgA产生和SCFA浓度之间的正相关，以及总日增重与哺乳仔猪肠道健康之间的正相关。综上所述，我们的研究结果表明，母体白藜芦醇可以促进肠道健康，从而在夏季高温期间改善仔猪生长，这可能与母猪初乳中的免疫球蛋白和源自外泌体的miRNA有关。
