Miura Hiroto, Jimbo Itsuki, Oda Machi, Noguchi Michiko, Kawasaki Kiyonori, Osada-Oka Mayuko, Tsukahara Takamitsu, Inoue Ryo
Laboratory of Animal Science, Setsunan University, Hirakata, Osaka 573-0101, Japan.
Laboratory of Animal Science, Kyoto Prefectural University, Kyoto, Kyoto 606-8522, Japan.
Animals (Basel). 2022 Aug 24;12(17):2172. doi: 10.3390/ani12172172.
Growing evidence indicates that porcine colostral exosomes may contribute to the healthy development of piglets. Here, we evaluated in vitro the effect of porcine milk-derived exosomes, in particular colostral exosomes, on T cells in the peripheral blood of suckling piglets. A total of seven sows and thirteen suckling piglets were used. Peripheral blood mononuclear cells (PBMCs) from suckling piglets were cultured with or without milk-derived exosomes (control). Using flow cytometry, the proportion of each T cell subset in cultured PBMCs was analyzed three days post-incubation. PBMCs cultured with porcine colostral exosomes had a higher proportion of CD3CD4CD8 T cells (cytotoxic T cells; Tc) than the control. However, exosomes induced no increase in the Tc cell population in PBMC whose endocytosis was inhibited. We further measured the concentrations of cytokines in the culture supernatant. Exosome-treated PBMCs had a higher cytokine IL-2 concentration than the control. The present study demonstrated that porcine colostral exosomes could increase the Tc cell proportion in the peripheral blood of suckling piglets, with the underlying mechanism believed to be the stimulation of IL-2 production in PBMCs via endocytosis. Moreover, our results suggested that porcine colostral exosomes were involved in the development of cellular immunity in suckling piglets.
越来越多的证据表明，猪初乳外泌体可能有助于仔猪的健康发育。在此，我们在体外评估了猪乳源外泌体，特别是初乳外泌体，对哺乳仔猪外周血T细胞的影响。总共使用了7头母猪和13头哺乳仔猪。将哺乳仔猪的外周血单个核细胞（PBMC）在有或没有乳源外泌体（对照）的情况下进行培养。孵育三天后，使用流式细胞术分析培养的PBMC中每个T细胞亚群的比例。与对照相比，用猪初乳外泌体培养的PBMC中CD3CD4CD8 T细胞（细胞毒性T细胞；Tc）的比例更高。然而，外泌体并未使内吞作用受到抑制的PBMC中的Tc细胞群体增加。我们进一步测量了培养上清液中细胞因子的浓度。经外泌体处理的PBMC中细胞因子IL-2的浓度高于对照。本研究表明，猪初乳外泌体可增加哺乳仔猪外周血中Tc细胞的比例，其潜在机制被认为是通过内吞作用刺激PBMC中IL-2的产生。此外，我们的结果表明，猪初乳外泌体参与了哺乳仔猪细胞免疫的发育。
