Al-Rasheed Agharid Ali, Garba Bashiru, Handool Kareem Obayes, Al-Jashamy Karim Alwan, Odhah Mohamed Naji Ahmed, Dirie Najib Isse, Daud Hassan Mohd
Department of Microbiology, Faculty of Veterinary Medicine, Tikrit University, Tikrit, Iraq.
Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia.
Pan Afr Med J. 2023 Dec 21;46:112. doi: 10.11604/pamj.2023.46.112.38578. eCollection 2023.
due to the fact that antimicrobial peptides antimicrobial peptides (AMPs) from climbing perch have not been fully explored for their antimicrobial potency, this investigation was undertaken to explore that possibility.
antimicrobial peptides (AMPs) from the mucous secretion of climbing perch were obtained and an in-vivo analysis was conducted using mice.
the results showed inhibitory effects on multidrug-resistant multidrug-resistant Pseudomonas aeruginosa with reduced mortality from 100% among the non-treated group to 25%. Similarly, the level of serum transaminase enzymes (AST and ALT), creatinine levels, and pro-inflammatory cytokines (TNF-α and IL-6) were all found to be higher in the non-treatment group compared to the AMP-treatment group. Also, extensive tissue damage in the lung, liver, and spleen of the non-treated control group mice was observed based on the histopathological lesions recorded. As expected, AMPs from climbing perch significantly alleviated multidrug-resistant P. aeruginosa infection in-vivo and produced enhanced therapeutic efficacy superior to the ciprofloxacin treatment.
this study provides insight into the potential antimicrobial activity of fish innate immune system-derived peptides that could serve as a candidate for the substitute of antibiotics.
由于来自攀鲈的抗菌肽的抗菌效力尚未得到充分研究，因此进行了这项调查以探索这种可能性。
从攀鲈的粘液分泌物中获得抗菌肽，并使用小鼠进行体内分析。
结果显示对多重耐药铜绿假单胞菌有抑制作用，死亡率从未治疗组的100%降至25%。同样，与抗菌肽治疗组相比，未治疗组的血清转氨酶（AST和ALT）水平、肌酐水平和促炎细胞因子（TNF-α和IL-6）均更高。此外，根据记录的组织病理学病变，观察到未治疗对照组小鼠的肺、肝和脾有广泛的组织损伤。正如预期的那样，来自攀鲈的抗菌肽在体内显著减轻了多重耐药铜绿假单胞菌感染，并产生了优于环丙沙星治疗的增强治疗效果。
本研究深入了解了鱼类先天免疫系统衍生肽的潜在抗菌活性，这些肽可作为抗生素替代品的候选物。
