Khombi Shooshtari Maryam, Sarkaki Alireza, Rashno Mohammad, Hoseinynejad Khojasteh
Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Physiology, Faculty of Medicine, Persian Gulf Physiology Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Vet Res Forum. 2024;15(2):105-111. doi: 10.30466/vrf.2023.2000658.3859. Epub 2024 Feb 15.
Studies conducted on animal models have shown that the administration of glycerol can lead to kidney tissue damage and impaired renal function. This is believed to be caused by oxidative stress and inflammation, which in turn can result in elevated levels of blood urea nitrogen (BUN) and creatinine. These metabolites are commonly used as indicators of renal function. The aim of the current experimental research was to investigate the protective efficacy of ellagic acid in a rat model of rhabdomyolysis induced by glycerol. Sixty healthy adult male rats weighing between 250 - 300 g were divided into five equal groups including control, rhabdomyolysis (administered 8.00 mL kg of glycerol), and three rhabdomyolysis plus various doses of ellagic acid (25.00, 50.00 and 100 mg kg day; 72 hr after receiving glycerol for 14 days successively) groups. Serum levels of BUN, creatinine, lactate dehydrogenase, alkaline phosphatase, electrolytes and inflammatory cytokines were evaluated in all rats. Histopathological studies were also performed on kidney tissues from all groups. The administration of ellagic acid resulted in a significant increase in renal function biomarkers compared to the rats with acute kidney injury. This increase was consistent with notable reductions in tumor necrosis factor-α levels and increases in interleukin-10 levels observed in blood samples. Furthermore, the improvement in histopathological indices observed in rats received ellagic acid confirmed its nephroprotective role. The results of the current experimental study suggest that ellagic acid can improve kidney damage following glycerol injection, potentially by modulating the inflammatory process.
在动物模型上进行的研究表明，给予甘油会导致肾组织损伤和肾功能受损。据信这是由氧化应激和炎症引起的，进而会导致血尿素氮（BUN）和肌酐水平升高。这些代谢产物通常用作肾功能指标。当前实验研究的目的是研究鞣花酸在甘油诱导的大鼠横纹肌溶解模型中的保护作用。将60只体重在250 - 300克之间的健康成年雄性大鼠分为五组，包括对照组、横纹肌溶解组（给予8.00 mL/kg甘油）以及三组横纹肌溶解加不同剂量鞣花酸组（25.00、50.00和100 mg/kg/天；在连续接受甘油14天后72小时给药）。评估所有大鼠的血清BUN、肌酐、乳酸脱氢酶、碱性磷酸酶、电解质和炎性细胞因子水平。还对所有组的肾组织进行了组织病理学研究。与急性肾损伤大鼠相比，给予鞣花酸导致肾功能生物标志物显著增加。这种增加与血样中肿瘤坏死因子-α水平显著降低和白细胞介素-10水平升高一致。此外，接受鞣花酸的大鼠组织病理学指标的改善证实了其肾保护作用。当前实验研究结果表明，鞣花酸可能通过调节炎症过程来改善甘油注射后的肾损伤。
