Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091,China.
Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520,China.
Fitoterapia. 2024 Jun;175:105901. doi: 10.1016/j.fitote.2024.105901. Epub 2024 Mar 11.
To compare the bioactive compounds in agarwood induced by different methods in Aquilaria sinensis(Lour.) Gilg trees, a two dimensional thin layer chromatograph(2D-TLC) combined with effect directive analysis(EDA) was developed. Three antioxidants were found by 2D-TLC-DPPH and further identified as 2-(2-phenylethyl) chromones(PECs) with LC-MS/MS. The 3 antioxidants decreased along agarwood formation and their compositions in drilling induced agarwood differed with those in microbe culture induced agarwood. Further study showed NaCl treatment promoted antioxidants accumulation in agarwood induced by drilling or hot drilling. Hot drilling combined with salty stimulation was most efficient in some chemicals accumulation, which were identified as PECs with antioxidant, tyrosinase or β-glucosidase inhibiting activities by 2D-TLC-EDA-LC-MS/MS. This study provided a 2D-TLC-EDA-LC-MS/MS method for bioactive compounds screen and qualification of agarwood. Based on this method, non-conventional methods were found to accelerate the accumulation of some bioactive PECs in A. sinensis trees.
为了比较不同方法诱导的沉香木中生物活性化合物，开发了二维薄层色谱（2D-TLC）结合效应定向分析（EDA）。通过 2D-TLC-DPPH 发现了三种抗氧化剂，并用 LC-MS/MS 进一步鉴定为 2-（2-苯乙基）色酮（PECs）。这 3 种抗氧化剂随着沉香木的形成而减少，其在钻孔诱导沉香木中的组成与微生物培养诱导沉香木中的组成不同。进一步的研究表明，NaCl 处理促进了钻孔或热钻诱导沉香木中抗氧化剂的积累。热钻结合盐刺激在某些化学物质的积累方面最为有效，这些化学物质通过 2D-TLC-EDA-LC-MS/MS 被鉴定为具有抗氧化、酪氨酸酶或β-葡萄糖苷酶抑制活性的 PECs。本研究提供了一种 2D-TLC-EDA-LC-MS/MS 方法，用于筛选和鉴定沉香木中的生物活性化合物。基于该方法，发现了一些非传统方法可以加速沉香树中某些生物活性 PEC 的积累。
