具有有序排列结构的3D打印硅钙硼石支架通过诱导嵌体移植物中的巨噬细胞极化来促进骨再生。

3D-printed bredigite scaffolds with ordered arrangement structures promote bone regeneration by inducing macrophage polarization in onlay grafts.

作者信息

Xuan Yaowei, Guo Yibo, Li Lin, Zhang Chenping, Yin Xuelai, Zhang Zhen

机构信息

State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Department of Periodontology, School of Stomatology, National Clinical Research Center for Oral Diseases, Shaanxi International Joint Research Center for Oral Diseases, The Fourth Military Medical University, Xi'an, 710032, China.

Department of Oral & Maxillofacial-Head & Neck Oncology, Shanghai Ninth People's Hospital, College of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China.

出版信息

J Nanobiotechnology. 2024 Mar 11;22(1):102. doi: 10.1186/s12951-024-02362-2.

DOI:10.1186/s12951-024-02362-2

PMID:38468312

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10926610/

Abstract

Bone tissue engineering scaffolds may provide a potential strategy for onlay bone grafts for oral implants. For determining the fate of scaffold biomaterials and osteogenesis effects, the host immune response is crucial. In the present study, bredigite (BRT) bioceramic scaffolds with an ordered arrangement structure (BRT-O) and a random morphology (BRT-R) were fabricated. The physicochemical properties of scaffolds were first characterized by scanning electron microscopy, mechanical test and micro-Fourier transform infrared spectroscopy. In addition, their osteogenic and immunomodulatory properties in an onlay grafting model were investigated. In vitro, the BRT-O scaffolds facilitated the macrophage polarization towards a pro-regenerative M2 phenotype, which subsequently facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells. In vivo, an onlay grafting model was successfully established in the cranium of rabbits. In addition, the BRT-O scaffolds grafted on rabbit cranium promoted bone regeneration and CD68 + CD206 + M2 macrophage polarization. In conclusion, the 3D-printed BRT-O scaffold presents as a promising scaffold biomaterial for onlay grafts by regulating the local immune microenvironment.

摘要

骨组织工程支架可为口腔种植体的贴骨移植提供一种潜在策略。为了确定支架生物材料的转归和成骨效果，宿主免疫反应至关重要。在本研究中，制备了具有有序排列结构（BRT-O）和随机形态（BRT-R）的钙钛矿（BRT）生物陶瓷支架。首先通过扫描电子显微镜、力学测试和显微傅里叶变换红外光谱对支架的物理化学性质进行了表征。此外，还研究了它们在贴骨移植模型中的成骨和免疫调节特性。在体外，BRT-O支架促进巨噬细胞向促再生的M2表型极化，随后促进骨髓间充质干细胞的迁移和成骨分化。在体内，在兔颅骨中成功建立了贴骨移植模型。此外，移植到兔颅骨上的BRT-O支架促进了骨再生和CD68+CD206+M2巨噬细胞极化。总之，通过调节局部免疫微环境，3D打印的BRT-O支架是一种很有前景的贴骨移植支架生物材料。