Zhang Beibei, Jia Chenzheng, Li Mingyu, Wang Kai, Chen Jun, Zhao Jing
College of Ocean and Earth Sciences, Xiamen University, Xaimen, Fujian, China.
Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration (USER), Xiamen University, Xiamen, Fujian, China.
Front Microbiol. 2024 Feb 26;15:1268813. doi: 10.3389/fmicb.2024.1268813. eCollection 2024.
Bacterial outer membrane vesicles (OMVs) contain a variety of chemical compounds and play significant roles in maintaining symbiotic relationships in a changing ocean, but little is known about their function, particularly in sponge larval development. During the growth of sponge sp., OMVs from species significantly promoted larval settlement, and SP-7-OMVs were selected as a representative strain for further investigation. According to OMVs metabolomics, larval settlement might be connected to organic acids and derivatives. The multiomics analysis of the genome, SP-7-OMVs metabolome, and larval transcriptome revealed 47 shared KEGG pathways. Among the number of candidate metabolites, arginine was chosen for its greater ability to increase the settlement rate and its role as the principal substrate for nitric oxide (NO) synthesis of sponge larvae. In summary, these results demonstrated that sponge-associated bacteria might utilize OMVs and their cargo to support host development and make up for host metabolic pathway deficiencies. This study enhances our fundamental knowledge of OMVs in interactions between metazoan hosts and microorganisms that are crucial in the coevolution of marine ecosystems and the complex marine environment.
细菌外膜囊泡（OMVs）含有多种化合物，在不断变化的海洋中维持共生关系方面发挥着重要作用，但人们对其功能了解甚少，尤其是在海绵幼虫发育方面。在海绵sp.的生长过程中，来自某物种的OMVs显著促进了幼虫的附着，并且选择SP - 7 - OMVs作为代表性菌株进行进一步研究。根据OMVs代谢组学分析，幼虫附着可能与有机酸及其衍生物有关。对该基因组、SP - 7 - OMVs代谢组和幼虫转录组的多组学分析揭示了47条共享的KEGG途径。在众多候选代谢物中，精氨酸因其具有更强的提高附着率的能力以及作为海绵幼虫一氧化氮（NO）合成的主要底物的作用而被选中。总之，这些结果表明，与海绵相关的细菌可能利用OMVs及其所载物质来支持宿主发育，并弥补宿主代谢途径的不足。这项研究增进了我们对后生动物宿主与微生物相互作用中OMVs的基础知识，这种相互作用在海洋生态系统的共同进化和复杂的海洋环境中至关重要。
