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单细胞分析和功能筛选揭示了长链非编码RNA在人类急性伤口表皮再上皮化中的关键作用。

Single-cell profiling and functional screening reveal crucial roles for lncRNAs in the epidermal re-epithelialization of human acute wounds.

作者信息

Xiao Yunting, Zhang Chenyang, Liu Xiuping, Yang Yong, Landén Ning Xu, Zhang Zhao, Li Dongqing

机构信息

Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.

Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China.

出版信息

Front Surg. 2024 Feb 26;11:1349135. doi: 10.3389/fsurg.2024.1349135. eCollection 2024.

DOI:10.3389/fsurg.2024.1349135
PMID:38468869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10925684/
Abstract

OBJECTIVES

Re-epithelialization is an important physiological process for repairing skin barrier function during wound healing. It is primarily mediated by coordinated migration, proliferation, and differentiation of keratinocytes. Long noncoding RNAs (lncRNAs) are essential components of the noncoding genome and participate in various biological processes; however, their expression profiles and function in re-epithelialization during wound healing have not been established.

METHODS

We investigated the distribution of lncRNAs during wound re-epithelialization by comparing the genomic profiles of uninjured skin and acute wound (AW) from healthy donors. We performed functional screening of differentially expressed lncRNAs to identify the important lncRNAs for re-epithelialization.

RESULTS

The expression of multiple lncRNAs is changed during human wound re-epithelialization process. We identified VIM-AS1, SMAD5-AS1, and LINC02581 as critical regulators involved in keratinocyte migration, proliferation, and differentiation, respectively.

CONCLUSION

LncRNAs play crucial regulatory roles in wound re-epithelialization. We established lncRNA expression profile in human acute wounds compared with intact skin, offering valuable insights into the physiological mechanisms underlying wound healing and potential therapeutic targets.

摘要

目的

重新上皮化是伤口愈合过程中修复皮肤屏障功能的重要生理过程。它主要由角质形成细胞的协调迁移、增殖和分化介导。长链非编码RNA（lncRNA）是非编码基因组的重要组成部分，参与各种生物学过程；然而，它们在伤口愈合过程中重新上皮化的表达谱和功能尚未明确。

方法

我们通过比较健康供体未受伤皮肤和急性伤口（AW）的基因组图谱，研究了lncRNA在伤口重新上皮化过程中的分布。我们对差异表达的lncRNA进行功能筛选，以鉴定对重新上皮化重要的lncRNA。

结果

在人类伤口重新上皮化过程中，多种lncRNA的表达发生变化。我们分别鉴定出VIM-AS1、SMAD5-AS1和LINC02581作为参与角质形成细胞迁移、增殖和分化的关键调节因子。

结论

lncRNA在伤口重新上皮化中起关键调节作用。我们建立了与完整皮肤相比的人类急性伤口lncRNA表达谱，为伤口愈合的生理机制和潜在治疗靶点提供了有价值的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/04e72042e84e/fsurg-11-1349135-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/f1b8307ae8a4/fsurg-11-1349135-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/c4547cf6fb04/fsurg-11-1349135-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/05c0e195622b/fsurg-11-1349135-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/2ab7f431dccc/fsurg-11-1349135-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/eb33fddcbe64/fsurg-11-1349135-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/04e72042e84e/fsurg-11-1349135-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/f1b8307ae8a4/fsurg-11-1349135-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/c4547cf6fb04/fsurg-11-1349135-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/05c0e195622b/fsurg-11-1349135-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/2ab7f431dccc/fsurg-11-1349135-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/eb33fddcbe64/fsurg-11-1349135-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4aab/10925684/04e72042e84e/fsurg-11-1349135-g006.jpg

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VIM‑AS1 promotes proliferation and drives enzalutamide resistance in prostate cancer via IGF2BP2‑mediated HMGCS1 mRNA stabilization.
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lncRNA-H19 in Fibroblasts Promotes Wound Healing in Diabetes.lncRNA-H19 在成纤维细胞中促进糖尿病伤口愈合。
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