DGUOK-AS1 通过抑制靶向 SPRR1B 的 miR-499a-5p 促进宫颈鳞癌细胞体外进展。
DGUOK-AS1 promotes cervical squamous cell carcinoma progression by suppressing miR-499a-5p that targets SPRR1B in vitro.
机构信息
Department of Obstetrics, Taikang Tongji (Wuhan) Hospital, Wuhan, 430050, China.
Department of Gynaecology, Shenzhen Baoan Shiyan People's Hospital, Shenzhen, 518108, China.
出版信息
Biochem Biophys Res Commun. 2021 Dec 31;585:177-184. doi: 10.1016/j.bbrc.2021.11.003. Epub 2021 Nov 6.
PURPOSE
Cervical squamous cell carcinoma (CESC) is the most common cancer type of cervical cancer, which threatens women's life seriously. LncRNA DGUOK-AS1has been reported to promote the biologic processes of CESC. We aim to figure out the role of DGUOK-AS1-miR-499a-5p-SPRR1B axis in modulating the CESC progression in vitro.
METHODS
The levels of DGUOK-AS1, miR-499a-5p, and SPRR1B in CESC tissues and cells were examined by RT-qPCR. The interaction of DGUOK-AS1-miR-499a-5p-SPRR1B was verified by luciferase assay. Inhibition of DGUOK-AS1, miR-499a-5p, and SPRR1B was applied for exploring the biological function based on detection of cell viability, proliferation, migration, and apoptosis in CESC SiHa and HeLa cells.
RESULTS
DGUOK-AS1 and SPRR1B expressions were obviously elevated, whereas the expression of miR-499a-5p was reduced in both CESC tissues and cells. Silencing of DGUOK-AS1 attenuated cell growth and boosted apoptosis of CESC cells. Notably, DGUOK-AS1 inhibited miR-499a-5p to release SPRR1B, which significantly accelerated the development of CESC.
CONCLUSION
DGUOK-AS1sponging miR-499a-5p facilitated CESC cells progression by releasing SPRR1B in vitro. It provides a new sight for the treatment of CESC patients involving DGUOK-AS1-miR-499a-5p-SPRR1B.
目的
宫颈鳞状细胞癌（CESC）是宫颈癌最常见的癌症类型，严重威胁着女性的生命。已有研究报道长链非编码 RNA（lncRNA）DGUK-AS1 可促进 CESC 的生物学进程。本研究旨在探讨 DGUK-AS1/miR-499a-5p/SRR1B 轴在体外调节 CESC 进展中的作用。
方法
采用 RT-qPCR 检测 CESC 组织和细胞中 DGUK-AS1、miR-499a-5p 和 SPRR1B 的水平。通过荧光素酶报告实验验证 DGUK-AS1/miR-499a-5p/SRR1B 的相互作用。应用 DGUK-AS1、miR-499a-5p 和 SPRR1B 抑制剂，检测 CESC SiHa 和 HeLa 细胞中的细胞活力、增殖、迁移和凋亡，以探讨其生物学功能。
结果
DGUK-AS1 和 SPRR1B 的表达明显上调，而 miR-499a-5p 的表达在 CESC 组织和细胞中均降低。DGUK-AS1 沉默可减弱 CESC 细胞的生长并促进其凋亡。值得注意的是，DGUK-AS1 抑制 miR-499a-5p 释放 SPRR1B，显著加速了 CESC 的发展。
结论
DGUK-AS1 通过海绵吸附 miR-499a-5p 释放 SPRR1B，促进 CESC 细胞在体外的进展。这为涉及 DGUK-AS1/miR-499a-5p-SRR1B 的 CESC 患者的治疗提供了新的思路。
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