Bai Wen Feng, Lin Zhe Guang, Yan Wei Yu, Zhang Li Zhen, Evans Jay D, Huang Qiang
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, China.
Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Jiangxi Agricultural University, Nanchang, China.
Front Insect Sci. 2021 Dec 3;1:756886. doi: 10.3389/finsc.2021.756886. eCollection 2021.
As a phoretic parasite and virus vector, the mite and the associated Deformed wing virus (DWV) form a lethal combination to the honey bee, . Routine acaricide treatment has been reported to reduce the diversity of mites and select for tolerance against these treatments. Further, different DWV strains face selective pressures when transmitted via mites. In this study, the haplotypes of mites and associated DWV variants were quantified using long reads. A single haplotype dominated the mite mitochondrial gene cytochrome oxidase subunit I, reflecting an ancient bottleneck. However, highly polymorphic genes were present across the mite genome, suggesting the diversity of mites could be actively maintained at a regional level. DWV detected in both mites and honey bees show a dominant variant with only a few low-frequency alternate haplotypes. The relative abundances of DWV haplotypes isolated from honey bees and mites were highly consistent, suggesting that some variants are favored by ongoing selection.
作为一种携播性寄生虫和病毒载体，瓦螨及其相关的残翅病毒（DWV）对蜜蜂构成了致命组合。据报道，常规杀螨剂处理会降低瓦螨的多样性，并促使其产生对这些处理的耐受性。此外，不同的DWV毒株在通过瓦螨传播时面临选择压力。在本研究中，利用长读长对瓦螨的单倍型及其相关的DWV变体进行了定量分析。单一单倍型在瓦螨线粒体基因细胞色素氧化酶亚基I中占主导地位，这反映了一个古老的瓶颈效应。然而，瓦螨基因组中存在高度多态性的基因，这表明瓦螨的多样性可能在区域水平上得到积极维持。在瓦螨和蜜蜂中检测到的DWV均显示出一个占主导地位的变体，只有少数低频替代单倍型。从蜜蜂和瓦螨中分离出的DWV单倍型的相对丰度高度一致，这表明一些变体受到持续选择的青睐。
