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自噬基因AMBRA1和ATG8在黄热病蚊子的中肠重塑中起关键作用。

Autophagy genes AMBRA1 and ATG8 play key roles in midgut remodeling of the yellow fever mosquito, .

作者信息

Albishi Najla M, Palli Subba Reddy

机构信息

Department of Entomology, University of Kentucky, Lexington, KY, United States.

出版信息

Front Insect Sci. 2023 Jan 16;3:1113871. doi: 10.3389/finsc.2023.1113871. eCollection 2023.

DOI:10.3389/finsc.2023.1113871
PMID:38469502
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10926384/
Abstract

The function of two autophagy genes, an activating molecule BECN1 regulated autophagy (AMBRA1) and autophagy-related gene 8 (ATG8) in the midgut remodeling of was investigated. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis of RNA samples collected from the last instar larvae and pupae showed that these two genes are predominantly expressed during the last 12 h and first 24 h of the last larval and pupal stages, respectively. Stable ecdysteroid analog induced and juvenile hormone (JH) analog suppressed these genes. RNA interference (RNAi) studies showed that the ecdysone-induced transcription factor E93 is required for the expression of these genes. JH-induced transcription factor krüppel homolog 1 (Kr-h1) suppressed the expression of these genes. RNAi-mediated silencing of and blocked midgut remodeling. Histological studies of midguts from insects at 48 h after ecdysis to the final larval stage and 12 h after ecdysis to the pupal stage showed that gene knockdown blocked midgut remodeling. and double-stranded (dsRNA)-treated insects retained larval midgut cells and died during the pupal stage. Together, these results demonstrate that ecdysteroid induction of genes initiates autophagy programmed cell death during midgut remodeling. JH inhibits midgut remodeling during metamorphosis by interfering with the expression of ATG genes.

摘要

研究了两个自噬基因，即激活分子BECN1调节自噬基因（AMBRA1）和自噬相关基因8（ATG8）在[昆虫名称未给出]中肠重塑过程中的作用。对从末龄幼虫和蛹收集的RNA样本进行实时定量聚合酶链反应（RT-qPCR）分析表明，这两个基因分别在末龄幼虫阶段的最后12小时和蛹期的最初24小时高表达。稳定的蜕皮甾类类似物诱导而保幼激素（JH）类似物抑制这些基因。RNA干扰（RNAi）研究表明，蜕皮激素诱导的转录因子E93是这些基因表达所必需的。JH诱导的转录因子克氏同源物1（Kr-h1）抑制这些基因的表达。RNAi介导的[基因名称未给出]和[基因名称未给出]沉默阻断了中肠重塑。对蜕皮至末龄幼虫阶段48小时和蜕皮至蛹期12小时的昆虫中肠进行组织学研究表明，[基因名称未给出]基因敲低阻断了中肠重塑。用[基因名称未给出]和[基因名称未给出]双链（dsRNA）处理的昆虫保留幼虫中肠细胞并在蛹期死亡。总之，这些结果表明蜕皮甾类对[基因名称未给出]基因的诱导在中肠重塑过程中启动了自噬程序性细胞死亡。JH通过干扰自噬相关基因（ATG）的表达来抑制变态过程中的中肠重塑。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b539/10926384/20902cc18256/finsc-03-1113871-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b539/10926384/bcc61784a967/finsc-03-1113871-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b539/10926384/42a4e5f29905/finsc-03-1113871-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b539/10926384/a9ff12eb6d25/finsc-03-1113871-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b539/10926384/20902cc18256/finsc-03-1113871-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b539/10926384/bcc61784a967/finsc-03-1113871-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b539/10926384/42a4e5f29905/finsc-03-1113871-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b539/10926384/a9ff12eb6d25/finsc-03-1113871-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b539/10926384/20902cc18256/finsc-03-1113871-g004.jpg

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