Division of Biotechnology, Department of Agronomy and Plant Breeding, Agricultural and Natural Resources College, University of Tehran, Karaj, Iran.
Department of Agrobiotechnology, Agrarian Technological Institute, RUDN University, Moscow, Russia.
Sci Rep. 2024 Mar 12;14(1):6000. doi: 10.1038/s41598-024-56709-8.
Oriental poppy (Papaver orientale L.) belonging to the Papaveraceae family, has the capacity to synthesize a wide range of benzylisoquinoline alkaloids (BIAs). This experiment was conducted to investigate the effects of green and chemical copper oxide nanoparticles (CuO NPs) elicitors on oxidative stress and the BIAs biosynthesis pathway in the cell suspension culture of P. orientale. This research shows that both green and chemical CuO NPs at concentrations of 20 mg/L and 40 mg/L, induce oxidative stress in the cell suspension of P. orientale by increasing the production of HO and the activity of antioxidant enzymes. The comparison of treatments revealed that utilizing a lower concentration of CuO NPs (20 mg/L) and extending the duration of cell suspension incubation (up to 48 h) play a more influential role in inducing the expression of the BIAs biosynthesis pathway genes (PsWRKY, TYDC, SalSyn, SalR, SalAT, T6ODM, COR and CODM) and increasing the production of morphinan alkaloids (thebaine, codeine, and morphine). The overarching results indicate that the concentration of CuO NPs and the duration of cell treatment have a more significant impact than the nature of CuO NPs in inducing oxidative stress and stimulating the expression of the BIAs pathway genes.
东方罂粟（Papaver orientale L.）属于罂粟科，具有合成多种苯并菲啶生物碱（BIAs）的能力。本实验旨在研究绿色和化学氧化铜纳米粒子（CuO NPs）激发子对东方罂粟细胞悬浮培养物中氧化应激和 BIAs 生物合成途径的影响。研究表明，浓度为 20 mg/L 和 40 mg/L 的绿色和化学 CuO NPs 均可通过增加 HO 的产生和抗氧化酶的活性来诱导 P. orientale 细胞悬浮液中的氧化应激。处理比较表明，较低浓度的 CuO NPs（20 mg/L）和延长细胞悬浮培养时间（长达 48 h）在诱导 BIAs 生物合成途径基因（PsWRKY、TYDC、SalSyn、SalR、SalAT、T6ODM、COR 和 CODM）的表达和增加吗啡烷生物碱（蒂巴因、可待因和吗啡）的产生方面发挥更重要的作用。总的结果表明，CuO NPs 的浓度和细胞处理时间比 CuO NPs 的性质对诱导氧化应激和刺激 BIAs 途径基因的表达有更显著的影响。
