Altissimi Caterina, Roila Rossana, Ranucci David, Branciari Raffaella, Cai Dongjie, Paulsen Peter
Department of Veterinary Medicine, University of Perugia, 06121 Perugia, Italy.
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Foods. 2024 Feb 22;13(5):658. doi: 10.3390/foods13050658.
We studied the efficacy of different formulations of polyphenol extracts (mainly containing hydroxytyrosol and tyrosol) from olive mill vegetation water on the microflora on the surfaces of game meat cuts with high or low initial bacterial loads. Meat with a high microbial load (>5 Log cfu/g; mean value = 6.83 ± 0.45 standard deviation) was immersed for 10 or 60 sec into 25% and 10% solutions of microencapsulated freeze-dried and non-encapsulated polyphenolic extracts. Aerobic colony, , spp., and lactic acid bacteria counts were determined on treated samples compared to controls after 7 days of storage (in vacuum-packed conditions at +3 °C). Significant differences were registered only for aerobic colony count for a 10% liquid extract treatment (0.64 log reduction). In contrast, the dipping or immersion of game meat with low initial microbial loads (<5 Log cfu/g; mean value = 3.58 ± 0.72 standard deviation) in 10% solutions of the polyphenol extracts effectuated significant reductions in all bacteria counts ( < 0.002) at 7 and 14 days of storage for different extracts, independently from the application methods. The use of the extracts to inhibit bacterial growth in game meat should only be considered if a good hygienic baseline is guaranteed.
我们研究了来自橄榄油厂废水的不同配方多酚提取物（主要含羟基酪醇和酪醇）对初始细菌载量高或低的野味肉切块表面微生物群落的功效。将微生物载量高（>5 Log cfu/g；平均值 = 6.83 ± 0.45标准差）的肉浸入25%和10%的微囊化冻干和非微囊化多酚提取物溶液中10或60秒。在储存7天后（在+3°C真空包装条件下），测定处理后样品与对照相比的需氧菌落、 、 spp.和乳酸菌计数。仅在10%液体提取物处理的需氧菌落计数方面有显著差异（减少0.64个对数）。相比之下，将初始微生物载量低（<5 Log cfu/g；平均值 = 3.58 ± 0.72标准差）的野味肉浸入10%的多酚提取物溶液中，在储存7天和14天时，不同提取物的所有细菌计数均显著降低（<0.002），且与应用方法无关。只有在保证良好卫生基线的情况下，才应考虑使用提取物抑制野味肉中的细菌生长。
