Ratnayake Jithendra, Gould Maree, Ramesh Niranjan, Mucalo Michael, Dias George J
Department of Oral Science, Faculty of Dentistry, University of Otago, Dunedin 9016, New Zealand.
Department of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin 9054, New Zealand.
Materials (Basel). 2024 Feb 28;17(5):1107. doi: 10.3390/ma17051107.
Hydroxyapatite is widely used in bone implantation because of its similar mineral composition to natural bone, allowing it to serve as a biocompatible osteoconductive support. A bovine-derived hydroxyapatite (BHA) scaffold was developed through an array of defatting and deproteinization procedures. The BHA scaffold was substituted with fluoride ions using a modified sol-gel method to produce a bovine-derived fluorapatite (BFA) scaffold. Fourier-transform infrared spectroscopy and X-ray diffraction analysis showed that fluoride ions were successfully substituted into the BHA lattice. According to energy dispersive X-ray analysis, the main inorganic phases contained calcium and phosphorus with a fluoride ratio of ~1-2 wt%. Scanning electron microscopy presented a natural microporous architecture for the BFA scaffold with pore sizes ranging from ~200-600 μm. The BHA scaffold was chemically stable and showed sustained degradation in simulated-body fluid. Young's modulus and yield strength were superior in the BFA scaffold to BHA. In vitro cell culture studies showed that the BFA was biocompatible, supporting the proliferative growth of Saos-2 osteoblast cells and exhibiting osteoinductive features. This unique technique of producing hydroxyapatite from bovine bone with the intent of producing high performance biomedically targeted materials could be used to improve bone repair.
羟基磷灰石因其矿物成分与天然骨相似，被广泛应用于骨植入领域，使其能够作为生物相容性骨传导支架。通过一系列脱脂和脱蛋白程序开发了一种牛源羟基磷灰石（BHA）支架。采用改进的溶胶 - 凝胶法用氟离子替代BHA支架，以制备牛源氟磷灰石（BFA）支架。傅里叶变换红外光谱和X射线衍射分析表明，氟离子成功地取代进入了BHA晶格。根据能量色散X射线分析，主要无机相包含钙和磷，氟含量约为1 - 2 wt%。扫描电子显微镜显示BFA支架具有天然微孔结构，孔径范围约为200 - 600μm。BHA支架化学性质稳定，在模拟体液中显示出持续降解。BFA支架的杨氏模量和屈服强度优于BHA。体外细胞培养研究表明，BFA具有生物相容性，支持Saos - 2成骨细胞的增殖生长，并表现出骨诱导特性。这种从牛骨生产羟基磷灰石以制备高性能生物医学靶向材料的独特技术可用于改善骨修复。
