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驼峰羟磷灰石的制备、理化特性表征及其用于骨修复的生物相容性研究。

Development, physicochemical characterization and biocompatibility study of dromedary camel dentine derived hydroxyapatite for bone repair.

机构信息

Department of Prosthodontics and Dental Implantology, College of Dentistry, King Faisal University, Al-Ahsa, Saudi Arabia.

Department of Oral Science, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.

出版信息

PeerJ. 2023 Aug 3;11:e15711. doi: 10.7717/peerj.15711. eCollection 2023.

DOI:10.7717/peerj.15711
PMID:37551347
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10404400/
Abstract

This study aimed to produce hydroxyapatite from the dentine portion of camel teeth using a defatting and deproteinizing procedure and characterize its physicochemical and biocompatibility properties. Biowaste such as waste camel teeth is a valuable source of hydroxyapatite, the main inorganic constituent of human bone and teeth which is frequently used as bone grafts in the biomedical field. Fourier Transform infrared (FTIR), and micro-Raman spectroscopy confirmed the functional groups as-sociated with hydroxyapatite. X-ray diffraction (XRD) studies showed camel dentine-derived hydroxyapatite (CDHA) corresponded with hydroxyapatite spectra. Scanning electron micros-copy (SEM) demonstrated the presence of dentinal tubules measuring from 1.69-2.91 µm. The inorganic phases of CDHA were primarily constituted of calcium and phosphorus, with trace levels of sodium, magnesium, potassium, and strontium, according to energy dispersive X-ray analysis (EDX) and inductively coupled plasma mass spectrometry (ICP-MS). After 28 days of incubation in simulated body fluid (SBF), the pH of the CDHA scaffold elevated to 9.2. biocompatibility studies showed that the CDHA enabled Saos-2 cells to proliferate and express the bone marker osteonectin after 14 days of culture. For applications such as bone augmentation and filling bone gaps, CDHA offers a promising material. However, to evaluate the clinical feasibility of the CDHA, further studies are required.

摘要

本研究旨在通过脱脂和脱蛋白程序从骆驼牙齿的牙本质部分中生产羟基磷灰石，并对其理化性质和生物相容性进行表征。生物废料如废弃的骆驼牙齿是羟基磷灰石的宝贵来源，羟基磷灰石是人类骨骼和牙齿的主要无机成分，常用于生物医学领域的骨移植。傅里叶变换红外（FTIR）和微拉曼光谱证实了与羟基磷灰石相关的官能团。X 射线衍射（XRD）研究表明，骆驼牙本质衍生的羟基磷灰石（CDHA）与羟基磷灰石图谱相对应。扫描电子显微镜（SEM）显示存在牙本质小管，直径为 1.69-2.91 µm。根据能量色散 X 射线分析（EDX）和电感耦合等离子体质谱（ICP-MS），CDHA 的无机相主要由钙和磷组成，痕量的钠、镁、钾和锶。在模拟体液（SBF）中孵育 28 天后，CDHA 支架的 pH 值升高到 9.2。生物相容性研究表明，CDHA 能够使 Saos-2 细胞在培养 14 天后增殖并表达骨标志物骨桥蛋白。对于骨增强和填充骨间隙等应用，CDHA 提供了一种有前途的材料。然而，为了评估 CDHA 的临床可行性，需要进一步的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417f/10404400/9d778887f189/peerj-11-15711-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417f/10404400/9d778887f189/peerj-11-15711-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417f/10404400/a4b6b232b876/peerj-11-15711-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417f/10404400/1d0382e8b495/peerj-11-15711-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417f/10404400/e49b243be88a/peerj-11-15711-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417f/10404400/02b81d48c757/peerj-11-15711-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417f/10404400/91a7dc0652f1/peerj-11-15711-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/417f/10404400/9d778887f189/peerj-11-15711-g013.jpg

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