Department of Anatomy, School of Biomedical Sciences, University of Otago, Dunedin 9054, New Zealand.
Department of Oral Sciences, Faculty of Dentistry, University of Otago, Dunedin 9054, New Zealand.
Int J Biol Macromol. 2020 Oct 1;160:1009-1020. doi: 10.1016/j.ijbiomac.2020.05.269. Epub 2020 Jun 3.
Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone. In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA). However, the processing methodology rendered the material mechanically weak. The present study investigated the infiltration of chitosan (CS) into the bovine HA scaffolds (CSHA) to improve the mechanical properties of BHA. The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds. X-ray Diffraction study confirmed the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds. SEM and μCT analyses showed the CSHA scaffolds presented adequate porosity and an interconnected porous architecture required for cell migration and attachment. CSHA scaffolds presented good thermal, chemical and structural stability while demonstrating sustained biodegradability in simulated body fluid. CSHA scaffolds presented mechanical properties significantly higher than the BHA scaffolds. CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
羟基磷灰石（HA）来源于牛骨，因其作为肉类废物的丰富来源，以及在形态和矿物质组成上与人体骨骼的相似性，作为骨替代物引起了广泛关注。在我们之前的工作中，我们开发了一种从新西兰牛松质骨（BHA）制备异种移植物 HA 支架的简单且可重复的方法。然而，该加工方法使材料的机械性能变弱。本研究探讨了壳聚糖（CS）渗透到牛 HA 支架（CSHA）中以提高 BHA 的机械性能。红外光谱检测到 HA 和 CS 的特征官能团的存在证实了 CS 渗透到 BHA 支架中。X 射线衍射研究证实了 HA 和 CSHA 支架中均存在羟基磷灰石相。SEM 和 μCT 分析表明，CSHA 支架具有足够的孔隙率和细胞迁移和附着所需的相互连接的多孔结构。CSHA 支架具有良好的热稳定性、化学稳定性和结构稳定性，同时在模拟体液中表现出持续的生物降解性。CSHA 支架的机械性能明显高于 BHA 支架。CSHA 支架与 Saos-2 成骨细胞具有生物相容性，并且比 BHA 支架更能显著促进细胞增殖，表明其在骨组织工程中的潜力。
