Krupka Sontje, Hoffmann Anne, Jasaszwili Mariami, Dietrich Arne, Guiu-Jurado Esther, Klöting Nora, Blüher Matthias
Helmholtz Institute for Metabolic, Obesity and Vascular Research (HI-MAG), Helmholtz Zentrum München, University of Leipzig and University Hospital Leipzig, 04103 Leipzig, Germany.
Medical Department III-Endocrinology, Nephrology, Rheumatology, University of Leipzig Medical Center, 04103 Leipzig, Germany.
Int J Mol Sci. 2024 Mar 2;25(5):2908. doi: 10.3390/ijms25052908.
Since the emergence of coronavirus disease-19 (COVID-19) in 2019, it has been crucial to investigate the causes of severe cases, particularly the higher rates of hospitalization and mortality in individuals with obesity. Previous findings suggest that adipocytes may play a role in adverse COVID-19 outcomes in people with obesity. The impact of COVID-19 vaccination and infection on adipose tissue (AT) is currently unclear. We therefore analyzed 27 paired biopsies of visceral and subcutaneous AT from donors of the Leipzig Obesity BioBank that have been categorized into three groups (1: no infection/no vaccination; 2: no infection but vaccinated; 3: infected and vaccinated) based on COVID-19 antibodies to spike (indicating vaccination) and/or nucleocapsid proteins. We provide additional insights into the impact of COVID-19 on AT biology through a comprehensive histological transcriptome and serum proteome analysis. This study demonstrates that COVID-19 infection is associated with smaller average adipocyte size. The impact of infection on gene expression was significantly more pronounced in subcutaneous than in visceral AT and mainly due to immune system-related processes. Serum proteome analysis revealed the effects of the infection on circulating adiponectin, interleukin 6 (IL-6), and carbonic anhydrase 5A (CA5A), which are all related to obesity and blood glucose abnormalities.
自2019年冠状病毒病（COVID-19）出现以来，调查重症病例的病因至关重要，尤其是肥胖个体住院率和死亡率较高的情况。先前的研究结果表明，脂肪细胞可能在肥胖人群COVID-19不良结局中发挥作用。目前尚不清楚COVID-19疫苗接种和感染对脂肪组织（AT）的影响。因此，我们分析了来自莱比锡肥胖生物样本库供体的27对内脏和皮下脂肪组织活检样本，这些样本根据针对刺突蛋白（表明接种疫苗）和/或核衣壳蛋白的COVID-19抗体被分为三组（1：未感染/未接种疫苗；2：未感染但接种了疫苗；3：感染且接种了疫苗）。我们通过全面的组织学转录组和血清蛋白质组分析，进一步深入了解COVID-19对脂肪组织生物学的影响。这项研究表明，COVID-19感染与平均脂肪细胞尺寸较小有关。感染对基因表达的影响在皮下脂肪组织中比在内脏脂肪组织中更为明显，主要是由于与免疫系统相关的过程。血清蛋白质组分析揭示了感染对循环脂联素、白细胞介素6（IL-6）和碳酸酐酶5A（CA5A）的影响，这些都与肥胖和血糖异常有关。
