Yan Xu, Dong Yao, Gu Yawen, Cui Hao
College of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
College of Biology & Food Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
Molecules. 2024 Mar 3;29(5):1132. doi: 10.3390/molecules29051132.
During the life activities of microorganisms, a variety of secondary metabolites are produced, including antimicrobials and antitumor drugs, which are widely used in clinical practice. In addition to exploring new antibiotics, this makes it one of the research priorities of to effectively increase the yield of antibiotics in production strains by various means. Most antibiotic-producing strains have a variety of functional regulatory factors that regulate their growth, development, and secondary metabolite biosynthesis processes. Through the study of precursor substances in antibiotic biosynthesis, researchers have revealed the precursor biosynthesis process and the mechanism by which precursor synthesis regulators affect the biosynthesis of secondary metabolites, which can be used to obtain engineered strains with high antibiotic production. This paper summarizes the supply of antibiotic biosynthesis precursors and the progress of research on the role of regulators in the process of precursors in biosynthesis. This lays the foundation for the establishment of effective breeding methods to improve antibiotic yields through the manipulation of precursor synthesis genes and related regulators.
在微生物的生命活动过程中，会产生多种次级代谢产物，包括抗菌药物和抗肿瘤药物，这些产物在临床实践中被广泛应用。除了探索新型抗生素外，这使得通过各种手段有效提高生产菌株中抗生素的产量成为研究重点之一。大多数抗生素生产菌株具有多种功能调控因子，这些因子调节其生长、发育以及次级代谢产物的生物合成过程。通过对抗生素生物合成中前体物质的研究，研究人员揭示了前体生物合成过程以及前体合成调节因子影响次级代谢产物生物合成的机制，这可用于获得高抗生素产量的工程菌株。本文综述了抗生素生物合成前体的供应情况以及调节因子在生物合成过程中前体作用的研究进展。这为通过操纵前体合成基因和相关调节因子建立提高抗生素产量的有效育种方法奠定了基础。
