Jacques Danielle, Bkaily Ghassan
Department of Immunology and Cell Biology, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC J1H 5N4, Canada.
Nutrients. 2024 Mar 5;16(5):745. doi: 10.3390/nu16050745.
Endocardial endothelium (EE) is a layer of cells covering the cardiac cavities and modulates cardiomyocyte function. This cell type releases several cardioactive factors, including Angiotensin II (Ang II). This octopeptide is known to induce cardiac hypertrophy. However, whether this circulating factor also induces EE hypertrophy is not known. Taurine is known to prevent cardiac hypertrophy. Whether this endogenous antioxidant prevents the effect of Ang II on human EE (hEE) will be verified. Using quantitative fluorescent probe imaging for calcium and reactive oxygen species (ROS), our results show that Ang II induces (10 M, 48 h treatment) an increase in hEE cell (hEEC) volume and its nucleus. Pretreatment with 20 mM of taurine prevents morphological remodeling and increases intracellular calcium and ROS. These results suggest that the reported Ang II induces cardiac hypertrophy is associated with hEEC hypertrophy. This later effect is prevented by taurine by reducing intracellular calcium and ROS overloads. Thus, taurine could be an excellent tool for preventing Ang II-induced remodeling of hEECs.
心内膜内皮细胞（EE）是覆盖心脏腔室的一层细胞，可调节心肌细胞功能。这种细胞类型会释放多种心脏活性因子，包括血管紧张素II（Ang II）。已知这种八肽会诱导心肌肥大。然而，这种循环因子是否也会诱导EE肥大尚不清楚。已知牛磺酸可预防心肌肥大。这种内源性抗氧化剂是否能预防Ang II对人EE（hEE）的影响将得到验证。使用针对钙和活性氧（ROS）的定量荧光探针成像，我们的结果表明，Ang II（10 μM，处理48小时）会诱导hEE细胞（hEEC）体积及其细胞核增大。用20 mM牛磺酸预处理可防止形态重塑，并增加细胞内钙和ROS。这些结果表明，报道的Ang II诱导心肌肥大与hEEC肥大有关。牛磺酸通过减少细胞内钙和ROS过载来预防这种后期效应。因此，牛磺酸可能是预防Ang II诱导的hEEC重塑的极佳工具。
