The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
Int J Mol Sci. 2022 Jun 10;23(12):6518. doi: 10.3390/ijms23126518.
Aluminum (Al) toxicity is an essential factor that adversely limits soybean ( (L.) Merr.) growth in acid soils. WRKY transcription factors play important roles in soybean responses to abiotic stresses. Here, was screened from genes that were differentially expressed under Al treatment in Al-tolerant soybean Baxi10 and Al-sensitive soybean Bendi2. We found that was significantly induced by 20 μM AlCl and upregulated by AlCl treatment for 2 h. In different tissues, the expression of was differentially induced. In 0-1 cm root tips, the expression of was induced to the highest level. The overexpression of in soybean resulted in higher relative root elongation, root weight, depth, root length, volume, number of root tips and peroxidase activity but lower root average diameter, malonaldehyde and HO contents, indicating enhanced Al tolerance. Moreover, RNA-seq identified 205 upregulated and 108 downregulated genes in transgenic lines. Fifteen of these genes that were differentially expressed in both AlCl-treated and -overexpressing soybean had the W-box element, which can bind to the upstream-conserved WRKY domain. Overall, the combined functional analysis indicates that may improve soybean Al tolerance by regulating downstream genes participating in Al transport, organic acid secretion and antioxidant reactions.
铝（Al）毒性是限制大豆（（L.）Merr.）在酸性土壤中生长的一个重要因素。WRKY 转录因子在大豆应对非生物胁迫的过程中发挥着重要作用。在这里，我们从耐铝大豆 Baxi10 和铝敏感大豆 Bendi2 在铝处理下差异表达的基因中筛选出 。我们发现，20 μM AlCl 显著诱导 表达，并在 AlCl 处理 2 h 时上调表达。在不同的组织中， 的表达被不同程度地诱导。在 0-1 cm 的根尖中， 的表达被诱导到最高水平。在大豆中超表达 导致相对根伸长率、根重、根深度、根长、根体积、根尖数和过氧化物酶活性增加，而根平均直径、丙二醛和 H2O2 含量降低，表明增强了耐铝性。此外，RNA-seq 在 转基因系中鉴定出 205 个上调和 108 个下调基因。这些在 AlCl 处理和过表达大豆中差异表达的基因中有 15 个具有 W-box 元件，该元件可以与上游保守的 WRKY 结构域结合。总的来说，综合功能分析表明，可能通过调节参与 Al 转运、有机酸分泌和抗氧化反应的下游基因来提高大豆的耐铝性。
