Zhai Tingkai, Lan Shuoxian, Xv Luzhen, Zhang Xueying, Ma Xiangwei, Li Zhuoyun, Gao Jie, Chen Yukun, Lai Zhongxiong, Lin Yuling
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2024 Feb 28;13(5):662. doi: 10.3390/plants13050662.
The basic leucine zip (bZIP) transcription factors (TFs) are a group of highly conserved gene families that play important roles in plant growth and resistance to adversity stress. However, studies on hormonal regulatory pathways and functional analysis during somatic embryogenesis (SE) in is still unavailable. In this study, a total of 51 bZIP family members were systematically identified in the whole genome of longan, a comprehensive bioinformatics analysis of ( family members of longan) was performed, and subcellular localization and profiles patterns after transiently transformed were analyzed. The combined analysis of RNA-seq, ATAC-seq and ChIP-seq showed that four members have different H3K4me1 binding peaks in early SE and differentially expressed with increased chromatin accessibility. Comparative transcriptome analysis of bZIPs expression in early SE, different tissues and under 2,4-D treatment revealed that family might involved in growth and development during longan early SE. The qRT-PCR results implied that family were subjected to multiple hormonal responses and showed different degrees of up-regulated expression under indole-3-acetic acid (IAA), abscisic acid (ABA) and methyl jasmonate (MeJA) treatments, which indicated that they played an important role in the hormone synthesis pathways associated with the early SE of longan. Subcellular localization showed that DlbZIP60 was located in the nucleus, and the contents of endogenous IAA, MeJA and ABA were up-regulated in transiently overexpressed cell lines. These results suggest that may mediate hormones pathways that functions the development during early SE in longan.
基本亮氨酸拉链（bZIP）转录因子是一组高度保守的基因家族，在植物生长和逆境胁迫抗性中发挥重要作用。然而，关于其在龙眼体细胞胚胎发生（SE）过程中的激素调控途径和功能分析的研究仍然缺乏。在本研究中，在龙眼全基因组中系统鉴定出51个bZIP家族成员，对龙眼bZIP家族成员进行了全面的生物信息学分析，并分析了瞬时转化后的亚细胞定位和表达模式。RNA-seq、ATAC-seq和ChIP-seq的联合分析表明，四个成员在早期SE中有不同的H3K4me1结合峰，且随着染色质可及性增加而差异表达。对bZIPs在早期SE、不同组织以及2,4-D处理下的表达进行比较转录组分析，表明bZIP家族可能参与龙眼早期SE的生长和发育。qRT-PCR结果表明，bZIP家族受到多种激素响应，在吲哚-3-乙酸（IAA）、脱落酸（ABA）和茉莉酸甲酯（MeJA）处理下呈现不同程度的上调表达，这表明它们在与龙眼早期SE相关的激素合成途径中发挥重要作用。亚细胞定位显示DlbZIP60定位于细胞核，在瞬时过表达细胞系中内源性IAA、MeJA和ABA的含量上调。这些结果表明，bZIP可能介导了在龙眼早期SE发育过程中起作用的激素途径。
