Wahyuni Dinar S C, Klinkhamer Peter G L, Choi Young Hae, Leiss Kirsten A
Plant Science and Natural Products, Institute of Biology (IBL), Leiden University, Sylviusweg 72, 2333BE Leiden, The Netherlands.
Pharmacy Department, Faculty Mathematics and Natural Sciences, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta 57126, Indonesia.
Plants (Basel). 2024 Feb 29;13(5):687. doi: 10.3390/plants13050687.
The defense mechanisms of plants evolve as they develop. Previous research has identified chemical defenses against Western flower thrips (WFT) in ( L.). Consequently, our study aimed to explore the consistency of these defense variations against WFT across the various developmental stages of grown under different conditions. Thrips bioassays were conducted on whole plants at three developmental stages, using the Charming Beauty and Robinetta varieties as examples of susceptible and resistant varieties, respectively. Metabolomic profiles of the leaves, buds and flowers before thrips infestation were analyzed. The thrips damage in Charming Beauty was more than 500-fold higher than the damage in Robinetta at all plant development stages. Relative concentrations of triterpenoid saponins and amino acids that were associated with resistance were higher in Robinetta at all plant stages. In Charming Beauty, the leaves exhibited greater damage compared to buds and flowers. The relative concentrations of alanine, valine and threonine were higher in buds and flowers than in leaves. The Metabolomic profiles of the leaves did not change significantly during plant development. In addition, we cultivated plants under different environmental conditions, ensuring consistency in the performance of the two varieties across different growing conditions. In conclusion, the chemical thrips resistance markers, based on the analysis of vegetative plants grown in climate rooms, were consistent over the plant's lifetime and for plants grown under field conditions.
植物的防御机制随着其生长发育而演变。先前的研究已经确定了番茄（L.）对西花蓟马（WFT）的化学防御。因此，我们的研究旨在探讨在不同条件下生长的番茄各个发育阶段，这些针对西花蓟马的防御变化的一致性。以迷人美女（Charming Beauty）和罗比内塔（Robinetta）品种分别作为感病品种和抗病品种的例子，在三个发育阶段对整株植物进行蓟马生物测定。分析了蓟马侵染前叶片、芽和花的代谢组学特征。在所有植物发育阶段，迷人美女品种的蓟马损害比罗比内塔品种高出500多倍。在所有植物阶段，与抗性相关的三萜皂苷和氨基酸的相对浓度在罗比内塔品种中更高。在迷人美女品种中，叶片比芽和花表现出更大的损害。芽和花中丙氨酸、缬氨酸和苏氨酸的相对浓度高于叶片。叶片的代谢组学特征在植物发育过程中没有显著变化。此外，我们在不同环境条件下种植植物，确保两个品种在不同生长条件下的表现一致。总之，基于对在气候室中生长的营养植物的分析，化学蓟马抗性标记在植物的整个生命周期以及田间条件下生长的植物中都是一致的。
