Heo Seong-Ho, Kim Su-Yeon, Mo Suk-Yeon, Park Han-Yong
Department of Bioresources Engineering, Sejong University, Seoul 05006, Republic of Korea.
Institute of Breeding Research, DASAN Co., Ltd., Pyeongtaek 17864, Republic of Korea.
Plants (Basel). 2024 Mar 4;13(5):725. doi: 10.3390/plants13050725.
Radish ( L.), a root vegetable belonging to the Brassicaceae family, is considered one of the representative crops displaying sporophytic self-incompatibility (SSI). The utilization of a self-incompatibility system in F breeding can improve the efficiency of cross-combinations, leading to a reduction in breeding time and aiding in the development of novel F varieties. The successful implementation of this system necessitates the rapid and accurate identification of S haplotypes in parental lines. In this study, we identified a total of nine S haplotypes among 22 elite radish lines through Sanger sequencing. Subsequently, we obtained sequences for showing a 95% similarity to nine S haplotypes, along with sequences identified by other researchers using BLAST. Following this, multiple sequence alignment (MSA) was conducted to identify and sequence similarities, as well as polymorphisms within the class I and II groups. Subsequently, S haplotype-specific marker sets were developed, targeting polymorphic regions of and alleles. These markers successfully amplified each of the nine S haplotypes. These markers will play a crucial role in the rapid and precise identification of parental S haplotypes in the radish F breeding process, proving instrumental in the radish F purity test.
萝卜（L.）是十字花科的一种根菜类蔬菜，被认为是表现出孢子体自交不亲和性（SSI）的代表性作物之一。在F育种中利用自交不亲和系统可以提高杂交组合的效率，从而缩短育种时间并有助于培育新的F品种。该系统的成功实施需要快速准确地鉴定亲本系中的S单倍型。在本研究中，我们通过桑格测序在22个优良萝卜品系中总共鉴定出9种S单倍型。随后，我们获得了与9种S单倍型具有95%相似性的序列，以及其他研究人员使用BLAST鉴定的序列。在此之后，进行了多序列比对（MSA）以鉴定I类和II类组内的序列相似性以及多态性。随后，针对和等位基因的多态性区域开发了S单倍型特异性标记集。这些标记成功扩增了9种S单倍型中的每一种。这些标记将在萝卜F育种过程中亲本S单倍型的快速精确鉴定中发挥关键作用，对萝卜F纯度检测具有重要意义。
