Fujimoto Ryo, Sugimura Tetsu, Nishio Takeshi
Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
FEBS Lett. 2006 Jan 23;580(2):425-30. doi: 10.1016/j.febslet.2005.12.028. Epub 2005 Dec 19.
Self-compatible S-54 homozygotic plants were found in progenies of an F(1) hybrid cultivar in Chinese cabbage. Pollination tests revealed that this self-compatibility is controlled by the S locus and caused by the loss of the recognition function of the stigma. SRK, the gene for the recognition molecule in the stigma, was normally transcribed and translated in the self-compatible plants. The 1034-bp region in the receptor domain of SRK in the self-compatible plants was 100% identical to SLG in S-54, while that in self-incompatible S-54 homozygotic plants was 95.1% identical. These results suggest that the self-compatibility of the S-54 homozygotes is due to amino-acid changes caused by gene conversion from SLG to SRK.
在中国大白菜的一个F(1)杂交品种的后代中发现了自交亲和的S-54纯合植株。授粉试验表明，这种自交亲和性受S位点控制，是由柱头识别功能丧失引起的。SRK是柱头识别分子的基因，在自交亲和植株中能正常转录和翻译。自交亲和植株中SRK受体结构域的1034 bp区域与S-54中的SLG 100%相同，而在自交不亲和的S-54纯合植株中该区域的同源性为95.1%。这些结果表明，S-54纯合子的自交亲和性是由于基因从SLG转换为SRK导致的氨基酸变化所致。
