Department of Reproductive Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, 17 Yongwai Zhengjie, Nanchang, 330000, China.
Infection Control Department of the First Affiliated Hospital of Nanchang University, 17 Yongwai Zhengjie, Nanchang, 330000, China.
Eur J Med Res. 2024 Mar 12;29(1):162. doi: 10.1186/s40001-024-01755-1.
Active pulmonary tuberculosis (PTB) poses challenges in rapid diagnosis within complex clinical conditions. Given the close association between neutrophils and tuberculosis, we explored differentially expressed long non-coding RNAs (lncRNAs) in neutrophils as potential molecular markers for diagnosing active PTB. We employed a gene microarray to screen for lncRNA alterations in neutrophil samples from three patients with active PTB and three healthy controls. The results revealed differential expression of 1457 lncRNAs between the two groups, with 916 lncRNAs upregulated and 541 lncRNAs down-regulated in tuberculosis patients. Subsequent validation tests demonstrated down-regulation of lncRNA ZNF100-6:2 in patients with active PTB, which was restored following anti-tuberculosis treatment. Our findings further indicated a high diagnostic potential for lncRNA ZNF100-6:2, as evidenced by an area under the receiver operating characteristic (ROC) curve of 0.9796 (95% confidence interval: 0.9479 to 1.000; P < 0.0001). This study proposes lncRNA ZNF100-6:2 as a promising and novel diagnostic biomarker for active PTB.
活动性肺结核（PTB）在复杂的临床情况下快速诊断存在挑战。鉴于中性粒细胞与结核病之间的密切关联，我们探索了中性粒细胞中差异表达的长链非编码 RNA（lncRNA）作为诊断活动性 PTB 的潜在分子标志物。我们采用基因微阵列筛选了来自 3 例活动性 PTB 患者和 3 例健康对照者的中性粒细胞样本中的 lncRNA 改变。结果显示，两组之间有 1457 个 lncRNA 存在差异表达，其中结核病患者中有 916 个 lncRNA 上调，541 个 lncRNA 下调。随后的验证测试表明，lncRNA ZNF100-6:2 在活动性 PTB 患者中下调，经抗结核治疗后恢复。我们的研究结果进一步表明，lncRNA ZNF100-6:2 具有较高的诊断潜力，其受试者工作特征（ROC）曲线下面积为 0.9796（95%置信区间：0.9479 至 1.000；P<0.0001）。本研究提出 lncRNA ZNF100-6:2 作为一种有前途的新型活动性 PTB 诊断生物标志物。
