Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Department of Materials Science and Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
Methods Mol Biol. 2024;2783:269-278. doi: 10.1007/978-1-0716-3762-3_18.
Conventional therapies to address critically sized defects in subcutaneous adipose tissue remain a reconstructive challenge for surgeons, largely due to the lack of graft pre-vascularization. Adipose tissue relies on a dense microvasculature network to deliver nutrients, oxygen, nonadipose tissue-derived growth factors, cytokines, and hormones, as well as transporting adipose tissue-derived endocrine signals to other organ systems. This chapter addresses these vascularization issues by combining decellularized lung matrices with a step-wise seeding of patient-specific adipose-derived stem cells and endothelial cells to develop large-volume, perfusable, and pre-vascularized adipose grafts.
针对皮下脂肪组织中临界大小的缺损，传统的治疗方法仍然是外科医生面临的一项重建挑战，主要原因是移植物缺乏预先血管化。脂肪组织依赖于密集的微血管网络来输送营养物质、氧气、非脂肪组织衍生的生长因子、细胞因子和激素，并将脂肪组织衍生的内分泌信号输送到其他器官系统。本章通过将脱细胞肺基质与患者特异性脂肪来源干细胞和内皮细胞的逐步接种相结合，解决了这些血管生成问题，从而开发出大容量、可灌注和预先血管化的脂肪移植物。
