Department of Experimental Medicine, Histology and Embryology Section, University of Campania "L. Vanvitelli", Naples, Italy.
Methods Mol Biol. 2024;2777:83-89. doi: 10.1007/978-1-0716-3730-2_6.
Cancer stem cells (CSCs) are a small tumor cell subpopulation, driving cancer initiation, progression, multidrug resistance, and metastasis. Several methods are used to detect and isolate CSCs by flow cytometry. Among these, measurement of aldehyde dehydrogenase (ALDH) activity within the cell is an assay widely used to identify and isolate CSCs from different types of solid tumors. The aldehyde dehydrogenase (ALDH) is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids, overexpressed both in normal and cancer stem cells. In this chapter, it is described how CSCs are detected and isolated by using ALDH activity assay.
癌症干细胞（CSCs）是一小部分肿瘤细胞，驱动着癌症的发生、发展、多药耐药和转移。有几种方法可通过流式细胞术来检测和分离 CSCs。其中，细胞内醛脱氢酶（ALDH）活性的测量是一种广泛用于鉴定和分离不同类型实体瘤中 CSCs 的方法。醛脱氢酶（ALDH）是一种负责将醛氧化为羧酸的多态酶，在正常干细胞和癌症干细胞中均过度表达。在本章中，将描述如何通过 ALDH 活性测定来检测和分离 CSCs。
