Mele Luigi, Liccardo Davide, Tirino Virginia
Department of Experimental Medicine, Medical School, University of Campania "Luigi Vanvitelli", Naples, Italy.
Methods Mol Biol. 2018;1692:43-48. doi: 10.1007/978-1-4939-7401-6_4.
The aldehyde dehydrogenase (ALDH) is a polymorphic enzyme responsible for the oxidation of aldehydes to carboxylic acids. In this chapter, it is described the role of ALDH in the identification of cancer stem cells (CSCs), having been shown that stem cells express high levels of ALDH. Here, we present a method called ALDEFLUOR assay used for the identification, evaluation, and isolation of normal, cancer stem and progenitor cells.
醛脱氢酶（ALDH）是一种多态性酶，负责将醛氧化为羧酸。在本章中，将描述ALDH在癌症干细胞（CSC）鉴定中的作用，研究表明干细胞表达高水平的ALDH。在此，我们介绍一种称为ALDEFLUOR检测的方法，用于正常细胞、癌症干细胞和祖细胞的鉴定、评估及分离。
