Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research (CIBM), University of Granada, Granada, Spain.
Instituto de Investigación Biosanitaria ibs.GRANADA, University Hospitals of Granada-University of Granada, Granada, Spain.
Methods Mol Biol. 2024;2777:145-161. doi: 10.1007/978-1-0716-3730-2_11.
Cancer stem cells (CSCs) play a significant role in driving several tumor hallmarks. Their behavior and tumor progression are strictly related to the tumor microenvironment (TME). The dynamic interplay between CSCs and TME drives metastasis, chemoresistance, and disease relapse. In this chapter, we describe different techniques and protocols for isolating, culturing, and characterizing CSCs and we explain the methodology for the culture of multicellular spheroids comprising CSCs.
癌症干细胞 (CSCs) 在驱动多种肿瘤特征方面发挥着重要作用。它们的行为和肿瘤进展与肿瘤微环境 (TME) 密切相关。CSC 和 TME 之间的动态相互作用促使转移、化疗耐药和疾病复发。在本章中，我们描述了分离、培养和鉴定 CSCs 的不同技术和方案，并解释了包含 CSCs 的多细胞球体培养的方法学。
