College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, PR China; Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-Products Processing Technology of Shandong Province, Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, 23788 Gongye North Road, Jinan 250100, PR China.
Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture and Rural Affairs/Key Laboratory of Agro-Products Processing Technology of Shandong Province, Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, 23788 Gongye North Road, Jinan 250100, PR China.
Colloids Surf B Biointerfaces. 2024 May;237:113835. doi: 10.1016/j.colsurfb.2024.113835. Epub 2024 Mar 7.
The limited application of garlic essential oil (GEO) is attributed to its pungent taste, poor water solubility and low bioavailability. Liposomes are nontoxic, biodegradable and biocompatible, and β-cyclodextrin can inhibit undesirable odors and improve the stability and bioavailability. Thus a promising dual-layer GEO β-cyclodextrin inclusion compound liposome (GEO-DCL) delivery system with both advantages was designed and prepared in this study. Experimental results indicated that the encapsulation efficiency of GEO-DCLs was 5% higher than that of GEO liposomes (GEO-CLs), reaching more than 88%. In vitro release experiment showed that the release rate of GEO in GEO-DCLs was 40% lower than that of GEO-CLs after incubation in gastric juice for 6-h, indicating that the stability of GEO-DCLs was better than GEO-CLs. Evaluation of the effects of GEO-DCLs on lowering blood lipid levels in hypercholesterolemia mice. GEO-DCLs could reduce the weight and fat deposition in hypercholesterolemia mice. Inhibiting the increase of TC, LDL-C, and decrease of HDL-C in mice. The degree of liver injury was decreased, the number of round lipid droplets in liver cytoplasm was reduced, and the growth of fat cells was inhibited. The lipid-lowering effects of GEO-DCLs were dose-dependent. GEO-DCL can improve the bioavailability of GEO and improve dyslipidemia. Based on GEO's efficacy in lowering blood lipids, this study developed a kind of GEO-DCL compound pomegranate juice beverage with good taste, miscibility and double effect of reducing blood lipids. This study lays a foundation for the application of GEO in the field of functional food.
大蒜精油（GEO）的应用受到其刺激性气味、较差的水溶性和低生物利用度的限制。脂质体无毒、可生物降解且生物相容，β-环糊精可以抑制不良气味并提高稳定性和生物利用度。因此，本研究设计并制备了具有这两种优势的有前途的双层 GEO-β-环糊精包合物脂质体（GEO-DCL）给药系统。实验结果表明，GEO-DCL 的包封效率比 GEO 脂质体（GEO-CLs）高 5%，超过 88%。体外释放实验表明，在胃液孵育 6 小时后，GEO-DCLs 中 GEO 的释放率比 GEO-CLs 低 40%，表明 GEO-DCLs 的稳定性优于 GEO-CLs。评价 GEO-DCL 对降低高血脂小鼠血脂水平的影响。GEO-DCL 可以减轻高血脂小鼠的体重和脂肪沉积。抑制 TC、LDL-C 升高和 HDL-C 降低。肝损伤程度降低，肝细胞质中圆形脂滴数量减少，脂肪细胞生长受到抑制。GEO-DCL 的降血脂作用呈剂量依赖性。GEO-DCL 可以提高 GEO 的生物利用度并改善血脂异常。基于 GEO 降低血脂的功效，本研究开发了一种具有良好口感、混溶性和双重降血脂作用的 GEO-DCL 复合石榴汁饮料。本研究为 GEO 在功能性食品领域的应用奠定了基础。
