巯基乙醇在脱氢酶中NAD⁺-15-羟基前列腺素放射性薄层色谱分析中的作用。
Effect of mercaptoethanol in the radioactive thin layer chromatography assay of NAD+-15-hydroxyprostaglandin in dehydrogenase.
作者信息
Wright J T, Corder C N
出版信息
Prostaglandins. 1979 Mar;17(3):431-40. doi: 10.1016/s0090-6980(79)80011-8.
The use of mercaptoethanol in the assay of rat kidney 15-hydroxyprostaglandin dehydrogenase (PGDH) was found to have minimal effect on activity assayed with the spectrophotometric and substrate loss assays. However, mercaptoethanol appeared to inhibit PGDH when assayed by thin-layer chromatography, based upon conversion of 3H-PGE1 to 15-keto-3H-PGE1. Mercaptoethanol reacted with 15-keto-PGE1 to alter its chromatographic mobility and to suppress the U.V. absorption spectrum of 15-keto-PGE1. The implication of the use of ME in radiometric assays is discussed.
发现在用分光光度法和底物损失法测定大鼠肾脏15-羟基前列腺素脱氢酶（PGDH）的活性时，巯基乙醇的使用对活性影响极小。然而，基于3H-PGE1向15-酮-3H-PGE1的转化，在用薄层色谱法测定时，巯基乙醇似乎会抑制PGDH。巯基乙醇与15-酮-PGE1反应，改变其色谱迁移率并抑制15-酮-PGE1的紫外吸收光谱。讨论了在放射测定法中使用巯基乙醇的影响。
Zotero 插件