NAD⁺ - 15 - 羟基前列腺素脱氢酶在大鼠肾脏中的分布

NAD+-15-hydroxyprostaglandin dehydrogenase distribution in rat kidney.

作者信息

Wright J T, Corder C N

出版信息

J Histochem Cytochem. 1979 Feb;27(2):657-64. doi: 10.1177/27.2.448057.

DOI:10.1177/27.2.448057

Abstract

Rat kidney NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) was measured in zones and substructure of the rat kidney nephron. This was accomplished utilizing an assay procedure based upon determining the amount of prostaglandin E1 present before and after the reaction with the 15-hydroxyprostaglandin dehydrogenase contained in the tissue sample. The enzyme activity was assayed in freeze dried, quick frozen rat kidney sections and its distribution within the rat kidney was determined. In kidney zones, it was localized to medullary rays and inner cortex. In kidney substructure, activity was highest in collecting tubule, pars recti tubule, distal convoluted tubule and the ascending limb of Henle (14.2, 11.5, 6.4 and 9.2 mM kg-1hr-1, respectively). Activity in glomeruli, proximal convoluted tubule and small arteries was lower (2.1, 2.8 and 2.1 mM kg-1hr-1, respectively). The assay procedure was verified by established assays (spectrophotometric, fluorometric and radiometric TLC) which are often used in homogenate and purified PGDH preparations.

摘要

在大鼠肾单位的不同区域和亚结构中测定了大鼠肾脏NAD⁺依赖性15-羟基前列腺素脱氢酶（PGDH）。这是通过一种检测程序来完成的，该程序基于测定与组织样品中所含的15-羟基前列腺素脱氢酶反应前后前列腺素E1的含量。在冷冻干燥、速冻的大鼠肾脏切片中测定了该酶的活性，并确定了其在大鼠肾脏中的分布。在肾脏区域，它定位于髓放线和内皮质。在肾脏亚结构中，集合管、直部小管、远曲小管和髓袢升支的活性最高（分别为14.2、11.5、6.4和9.2 mM kg⁻¹hr⁻¹）。肾小球、近曲小管和小动脉中的活性较低（分别为2.1、2.8和2.1 mM kg⁻¹hr⁻¹）。该检测程序通过常用于匀浆和纯化PGDH制剂的既定检测方法（分光光度法、荧光法和放射性薄层色谱法）进行了验证。