Edmond and Lily Safra Center for Brain Sciences (ELSC), The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
ABBM, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Cell Rep. 2024 Mar 26;43(3):113943. doi: 10.1016/j.celrep.2024.113943. Epub 2024 Mar 13.
The maturation of engrams from recent to remote time points involves the recruitment of CA1 neurons projecting to the anterior cingulate cortex (CA1→ACC). Modifications of G-protein-coupled receptor pathways in CA1 astrocytes affect recent and remote recall in seemingly contradictory ways. To address this inconsistency, we manipulated these pathways in astrocytes during memory acquisition and tagged c-Fos-positive engram cells and CA1→ACC cells during recent and remote recall. The behavioral results were coupled with changes in the recruitment of CA1→ACC projection cells to the engram: Gq pathway activation in astrocytes caused enhancement of recent recall alone and was accompanied by earlier recruitment of CA1→ACC projecting cells to the engram. In contrast, Gi pathway activation in astrocytes resulted in the impairment of only remote recall, and CA1→ACC projecting cells were not recruited during remote memory. Finally, we provide a simple working model, hypothesizing that Gq and Gi pathway activation affect memory differently, by modulating the same mechanism: CA1→ACC projection.
从近期到远期时间点的记忆印痕成熟涉及到投射到前扣带皮层（CA1→ACC）的 CA1 神经元的募集。CA1 星形胶质细胞中的 G 蛋白偶联受体途径的修饰以看似矛盾的方式影响近期和远期回忆。为了解决这一不一致性，我们在记忆获取期间在星形胶质细胞中操纵这些途径，并在近期和远期回忆期间标记 c-Fos 阳性记忆印痕细胞和 CA1→ACC 细胞。行为结果与 CA1→ACC 投射细胞向记忆印痕的募集变化相关联：星形胶质细胞中的 Gq 途径激活仅导致近期回忆增强，并且伴随着 CA1→ACC 投射细胞更早地募集到记忆印痕。相比之下，星形胶质细胞中的 Gi 途径激活仅导致远期回忆受损，并且在远期记忆期间没有募集 CA1→ACC 投射细胞。最后，我们提供了一个简单的工作模型，假设 Gq 和 Gi 途径的激活通过调节相同的机制以不同的方式影响记忆：CA1→ACC 投射。
